Rui:TotoRNAseq protocol: Difference between revisions

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>Jdeng
>RuiLiu
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==totoRNA library structure==
==totoRNA library structure==
[[File:Libraries_ILMN.jpg]]
[[File:Libraries_ILMN.jpg]]
[[File:Libraries_totoRNA-5.jpg]]
[[File:Libraries_totoRNA-3.jpg]]


[[File:Libraries_totoRNA-5.jpg|600px]][[File:Libraries_totoRNA-3.jpg|600px]]
==totoRNA primer sequence==
* The 5' adaptors list
 
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''Tube ID'''
| align="center" style="background:#f0f0f0;"|'''Primer full name'''
| align="center" style="background:#f0f0f0;"|'''Primer full sequence'''
| align="center" style="background:#f0f0f0;"|'''PE_F-N10'''
| align="center" style="background:#f0f0f0;"|'''Barcode'''
| align="center" style="background:#f0f0f0;"|'''TSO'''
|-
| TSO.r01||BR_TSO_N10_BC6||ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNdUNNNNdUNNNTTTAGGrGrGrG||ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNN/ideoxyU/NNNN/ideoxyU/NNN||TTTAGG||rGrGrG
|-
| TSO.r02||B_TSO_N10||ACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG
|-
| TSO.r03||B.N10.TSO.NH2||ACACTCTTTCCCTACACGACGUNNNUNNNNUNNNrGrGrG/3AmMO/||ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG
|-
| TSO.id01||B.TSO.N10.BC8.Idx1||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTGAGACrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TCTGAGAC||rGrGrG
|-
| TSO.id02||B.TSO.N10.BC8.Idx2||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGCCCAAGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TGCCCAAG||rGrGrG
|-
| TSO.id03||B.TSO.N10.BC8.Idx3||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGACTCGCTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GACTCGCT||rGrGrG
|-
| TSO.id04||B.TSO.N10.BC8.Idx4||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCACTCCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CCACTCCA||rGrGrG
|-
| TSO.id05||B.TSO.N10.BC8.Idx5||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAACAGGGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GAACAGGG||rGrGrG
|-
| TSO.id06||B.TSO.N10.BC8.Idx6||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTGCTGATrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CTGCTGAT||rGrGrG
|-
| TSO.id07||B.TSO.N10.BC8.Idx7||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTAGTATCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CTAGTATC||rGrGrG
|-
| TSO.id08||B.TSO.N10.BC8.Idx8||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGCTGAAArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GGCTGAAA||rGrGrG
|-
| TSO.id09||B.TSO.N10.BC8.Idx9||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAGTGCTTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GAGTGCTT||rGrGrG
|-
| TSO.id10||B.TSO.N10.BC8.Idx10||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATACGCCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||ATACGCCA||rGrGrG
|-
| TSO.id11||B.TSO.N10.BC8.Idx11||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGCGTTArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||ATGCGTTA||rGrGrG
|-
| TSO.id12||B.TSO.N10.BC8.Idx12||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCACCCTTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TCACCCTT||rGrGrG
|-
| TSO.id13||B.TSO.N10.BC8.Idx13||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCAACCGCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CCAACCGC||rGrGrG
|-
| TSO.id14||B.TSO.N10.BC8.Idx14||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGATTACCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GGATTACC||rGrGrG
|-
| TSO.id15||B.TSO.N10.BC8.Idx15||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAGAGACArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CAGAGACA||rGrGrG
|-
| TSO.id16||B.TSO.N10.BC8.Idx16||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAATAGTGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CAATAGTG||rGrGrG
|-
| TSO.id17||B.TSO.N10.BC8.Idx17||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGGCCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GGTGGCCA||rGrGrG
|-
| TSO.id18||B.TSO.N10.BC8.Idx18||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGAGGGAGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GGAGGGAG||rGrGrG
|-
| TSO.id19||B.TSO.N10.BC8.Idx19||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTTGTCCTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GTTGTCCT||rGrGrG
|-
| TSO.id20||B.TSO.N10.BC8.Idx20||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCGACCTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TTCGACCT||rGrGrG
|-
| TSO.id21||B.TSO.N10.BC8.Idx21||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTTATTGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GCTTATTG||rGrGrG
|-
| TSO.id22||B.TSO.N10.BC8.Idx22||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAACCTTTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CAACCTTT||rGrGrG
|-
| TSO.id23||B.TSO.N10.BC8.Idx23||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTAGGTArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CTTAGGTA||rGrGrG
|-
| TSO.id24||B.TSO.N10.BC8.Idx24||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTCACCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GCTCACCA||rGrGrG
|-
| TSO.id25||B.TSO.N10.BC8.Idx25||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAAATGCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||AGAAATGC||rGrGrG
|-
| TSO.id26||B.TSO.N10.BC8.Idx26||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTACTGArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CTTACTGA||rGrGrG
|-
| TSO.id27||B.TSO.N10.BC8.Idx27||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTCACGGGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CTCACGGG||rGrGrG
|-
| TSO.id28||B.TSO.N10.BC8.Idx28||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACTGTCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CACTGTCA||rGrGrG
|-
| TSO.id29||B.TSO.N10.BC8.Idx29||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCTTGGGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TTCTTGGG||rGrGrG
|-
| TSO.id30||B.TSO.N10.BC8.Idx30||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNACCGGCTTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||ACCGGCTT||rGrGrG
|-
| TSO.id31||B.TSO.N10.BC8.Idx31||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTAACCACArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TAACCACA||rGrGrG
|-
| TSO.id32||B.TSO.N10.BC8.Idx32||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCGCGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TACGCGCG||rGrGrG
|-
| TSO.id33||B.TSO.N10.BC8.Idx33||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCTCGATGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CCTCGATG||rGrGrG
|-
| TSO.id34||B.TSO.N10.BC8.Idx34||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAGCTCArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||AGAGCTCA||rGrGrG
|-
| TSO.id35||B.TSO.N10.BC8.Idx35||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTATGGTCCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TATGGTCC||rGrGrG
|-
| TSO.id36||B.TSO.N10.BC8.Idx36||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGTCCGGCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TGTCCGGC||rGrGrG
|-
| TSO.id37||B.TSO.N10.BC8.Idx37||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAAGGAATCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||AAGGAATC||rGrGrG
|-
| TSO.id38||B.TSO.N10.BC8.Idx38||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCATCGTAGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CATCGTAG||rGrGrG
|-
| TSO.id39||B.TSO.N10.BC8.Idx39||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTGTTGAGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GTGTTGAG||rGrGrG
|-
| TSO.id40||B.TSO.N10.BC8.Idx40||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGTATCCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||ATGTATCC||rGrGrG
|-
| TSO.id41||B.TSO.N10.BC8.Idx41||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGACAGGTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TGACAGGT||rGrGrG
|-
| TSO.id42||B.TSO.N10.BC8.Idx42||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCCAArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TACGCCAA||rGrGrG
|-
| TSO.id43||B.TSO.N10.BC8.Idx43||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAAGGCAArGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GAAGGCAA||rGrGrG
|-
| TSO.id44||B.TSO.N10.BC8.Idx44||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACCCGAGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||CACCCGAG||rGrGrG
|-
| TSO.id45||B.TSO.N10.BC8.Idx45||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGTGTCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||GGTGTGTC||rGrGrG
|-
| TSO.id46||B.TSO.N10.BC8.Idx46||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTTGTGCrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||TCTTGTGC||rGrGrG
|-
| TSO.id47||B.TSO.N10.BC8.Idx47||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGTACCTGrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||AGTACCTG||rGrGrG
|-
| TSO.id48||B.TSO.N10.BC8.Idx48||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGCGTCCTrGrGrG||ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN||AGCGTCCT||rGrGrG
|-
| PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||||||
|}
 
* The 3' adaptor list
 
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''Tube ID'''
| align="center" style="background:#f0f0f0;"|'''Primer full name'''
| align="center" style="background:#f0f0f0;"|'''Primer full sequence'''
| align="center" style="background:#f0f0f0;"|'''PE_R'''
| align="center" style="background:#f0f0f0;"|'''Barcode'''
| align="center" style="background:#f0f0f0;"|'''T20VN'''
|-
| T20.r01||T20VN_PE_R||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||\||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.r02||N9_PE_R||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTNNNNNNNNN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||\||NNNNNNNNN
|-
| T20.r03||T20VN_BC6_PE_R||GCATTCCTGCTGAACCGCTCTTTTTAGGTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTT||TTTAGG||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id01||T20VN.BC8.PE_R.Indx1||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TCTGAGAC||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id02||T20VN.BC8.PE_R.Indx2||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TGCCCAAG||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id03||T20VN.BC8.PE_R.Indx3||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GACTCGCT||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id04||T20VN.BC8.PE_R.Indx4||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CCACTCCA||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id05||T20VN.BC8.PE_R.Indx5||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GAACAGGG||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id06||T20VN.BC8.PE_R.Indx6||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CTGCTGAT||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id07||T20VN.BC8.PE_R.Indx7||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CTAGTATC||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id08||T20VN.BC8.PE_R.Indx8||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GGCTGAAA||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id09||T20VN.BC8.PE_R.Indx9||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GAGTGCTT||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id10||T20VN.BC8.PE_R.Indx10||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||ATACGCCA||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id11||T20VN.BC8.PE_R.Indx11||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||ATGCGTTA||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id12||T20VN.BC8.PE_R.Indx12||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TCACCCTT||TTTTTTTTTTTTTTTTTTTTVN
|-
| T20.id13||T20V.BC8.PE_R.Indx13||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CCAACCGC||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id14||T20V.BC8.PE_R.Indx14||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GGATTACC||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id15||T20V.BC8.PE_R.Indx15||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CAGAGACA||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id16||T20V.BC8.PE_R.Indx16||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CAATAGTG||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id17||T20V.BC8.PE_R.Indx17||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GGTGGCCA||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id18||T20V.BC8.PE_R.Indx18||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GGAGGGAG||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id19||T20V.BC8.PE_R.Indx19||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GTTGTCCT||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id20||T20V.BC8.PE_R.Indx20||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TTCGACCT||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id21||T20V.BC8.PE_R.Indx21||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GCTTATTG||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id22||T20V.BC8.PE_R.Indx22||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CAACCTTT||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id23||T20V.BC8.PE_R.Indx23||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||CTTAGGTA||TTTTTTTTTTTTTTTTTTTTV
|-
| T20.id24||T20V.BC8.PE_R.Indx24||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||GCTCACCA||TTTTTTTTTTTTTTTTTTTTV
|-
| N2.id73||PCR_R.N2Ind73||CAAGCAGAAGACGGCATACGAGATGAGTCCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GAGTCC||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id74||PCR_R.N2Ind74||CAAGCAGAAGACGGCATACGAGATTCGCCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TCGCCA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id75||PCR_R.N2Ind75||CAAGCAGAAGACGGCATACGAGATAAGTCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AAGTCG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id76||PCR_R.N2Ind76||CAAGCAGAAGACGGCATACGAGATAATAGGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AATAGG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id77||PCR_R.N2Ind77||CAAGCAGAAGACGGCATACGAGATACCCGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCCGT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id78||PCR_R.N2Ind78||CAAGCAGAAGACGGCATACGAGATAACACGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AACACG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id79||PCR_R.N2Ind79||CAAGCAGAAGACGGCATACGAGATGCTTGGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCTTGG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id80||PCR_R.N2Ind80||CAAGCAGAAGACGGCATACGAGATTTACCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TTACCA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id81||PCR_R.N2Ind81||CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCAGGT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id82||PCR_R.N2Ind82||CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CGTTTG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id83||PCR_R.N2Ind83||CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GACCAC||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id84||PCR_R.N2Ind84||CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACAAGA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id85||PCR_R.N2Ind85||CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCGCA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id86||PCR_R.N2Ind86||CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGGGTA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id87||PCR_R.N2Ind87||CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ATTCCG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id88||PCR_R.N2Ind88||CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GAATGT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id89||PCR_R.N2Ind89||CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCTGAT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id90||PCR_R.N2Ind90||CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AGTGCT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id91||PCR_R.N2Ind91||CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CAGGGA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id92||PCR_R.N2Ind92||CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CATGCG||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id93||PCR_R.N2Ind93||CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGCCTA||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id94||PCR_R.N2Ind94||CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CTATAC||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id95||PCR_R.N2Ind95||CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCGAGT||CTCGGCATTCCTGCTGAACCGCTCTT
|-
| N2.id96||PCR_R.N2Ind96||CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCTGC||CTCGGCATTCCTGCTGAACCGCTCTT
|}

Revision as of 22:26, 26 November 2012

totoRNA library structure

File:Libraries ILMN.jpg File:Libraries totoRNA-5.jpg File:Libraries totoRNA-3.jpg

totoRNA primer sequence

  • The 5' adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r01 BR_TSO_N10_BC6 ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNdUNNNNdUNNNTTTAGGrGrGrG ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNN/ideoxyU/NNNN/ideoxyU/NNN TTTAGG rGrGrG
TSO.r02 B_TSO_N10 ACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r03 B.N10.TSO.NH2 ACACTCTTTCCCTACACGACGUNNNUNNNNUNNNrGrGrG/3AmMO/ ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.id01 B.TSO.N10.BC8.Idx1 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTGAGACrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCTGAGAC rGrGrG
TSO.id02 B.TSO.N10.BC8.Idx2 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGCCCAAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGCCCAAG rGrGrG
TSO.id03 B.TSO.N10.BC8.Idx3 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGACTCGCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GACTCGCT rGrGrG
TSO.id04 B.TSO.N10.BC8.Idx4 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCACTCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCACTCCA rGrGrG
TSO.id05 B.TSO.N10.BC8.Idx5 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAACAGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAACAGGG rGrGrG
TSO.id06 B.TSO.N10.BC8.Idx6 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTGCTGATrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTGCTGAT rGrGrG
TSO.id07 B.TSO.N10.BC8.Idx7 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTAGTATCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTAGTATC rGrGrG
TSO.id08 B.TSO.N10.BC8.Idx8 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGCTGAAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGCTGAAA rGrGrG
TSO.id09 B.TSO.N10.BC8.Idx9 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAGTGCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAGTGCTT rGrGrG
TSO.id10 B.TSO.N10.BC8.Idx10 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATACGCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATACGCCA rGrGrG
TSO.id11 B.TSO.N10.BC8.Idx11 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGCGTTArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATGCGTTA rGrGrG
TSO.id12 B.TSO.N10.BC8.Idx12 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCACCCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCACCCTT rGrGrG
TSO.id13 B.TSO.N10.BC8.Idx13 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCAACCGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCAACCGC rGrGrG
TSO.id14 B.TSO.N10.BC8.Idx14 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGATTACCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGATTACC rGrGrG
TSO.id15 B.TSO.N10.BC8.Idx15 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAGAGACArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAGAGACA rGrGrG
TSO.id16 B.TSO.N10.BC8.Idx16 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAATAGTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAATAGTG rGrGrG
TSO.id17 B.TSO.N10.BC8.Idx17 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGGCCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGTGGCCA rGrGrG
TSO.id18 B.TSO.N10.BC8.Idx18 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGAGGGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGAGGGAG rGrGrG
TSO.id19 B.TSO.N10.BC8.Idx19 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTTGTCCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GTTGTCCT rGrGrG
TSO.id20 B.TSO.N10.BC8.Idx20 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCGACCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TTCGACCT rGrGrG
TSO.id21 B.TSO.N10.BC8.Idx21 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTTATTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GCTTATTG rGrGrG
TSO.id22 B.TSO.N10.BC8.Idx22 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCAACCTTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CAACCTTT rGrGrG
TSO.id23 B.TSO.N10.BC8.Idx23 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTAGGTArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTTAGGTA rGrGrG
TSO.id24 B.TSO.N10.BC8.Idx24 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGCTCACCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GCTCACCA rGrGrG
TSO.id25 B.TSO.N10.BC8.Idx25 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAAATGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGAAATGC rGrGrG
TSO.id26 B.TSO.N10.BC8.Idx26 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTTACTGArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTTACTGA rGrGrG
TSO.id27 B.TSO.N10.BC8.Idx27 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCTCACGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CTCACGGG rGrGrG
TSO.id28 B.TSO.N10.BC8.Idx28 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACTGTCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CACTGTCA rGrGrG
TSO.id29 B.TSO.N10.BC8.Idx29 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTTCTTGGGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TTCTTGGG rGrGrG
TSO.id30 B.TSO.N10.BC8.Idx30 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNACCGGCTTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ACCGGCTT rGrGrG
TSO.id31 B.TSO.N10.BC8.Idx31 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTAACCACArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TAACCACA rGrGrG
TSO.id32 B.TSO.N10.BC8.Idx32 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCGCGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TACGCGCG rGrGrG
TSO.id33 B.TSO.N10.BC8.Idx33 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCCTCGATGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CCTCGATG rGrGrG
TSO.id34 B.TSO.N10.BC8.Idx34 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGAGCTCArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGAGCTCA rGrGrG
TSO.id35 B.TSO.N10.BC8.Idx35 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTATGGTCCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TATGGTCC rGrGrG
TSO.id36 B.TSO.N10.BC8.Idx36 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGTCCGGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGTCCGGC rGrGrG
TSO.id37 B.TSO.N10.BC8.Idx37 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAAGGAATCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AAGGAATC rGrGrG
TSO.id38 B.TSO.N10.BC8.Idx38 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCATCGTAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CATCGTAG rGrGrG
TSO.id39 B.TSO.N10.BC8.Idx39 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGTGTTGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GTGTTGAG rGrGrG
TSO.id40 B.TSO.N10.BC8.Idx40 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNATGTATCCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN ATGTATCC rGrGrG
TSO.id41 B.TSO.N10.BC8.Idx41 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTGACAGGTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TGACAGGT rGrGrG
TSO.id42 B.TSO.N10.BC8.Idx42 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTACGCCAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TACGCCAA rGrGrG
TSO.id43 B.TSO.N10.BC8.Idx43 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGAAGGCAArGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GAAGGCAA rGrGrG
TSO.id44 B.TSO.N10.BC8.Idx44 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNCACCCGAGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN CACCCGAG rGrGrG
TSO.id45 B.TSO.N10.BC8.Idx45 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNGGTGTGTCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN GGTGTGTC rGrGrG
TSO.id46 B.TSO.N10.BC8.Idx46 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNTCTTGTGCrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN TCTTGTGC rGrGrG
TSO.id47 B.TSO.N10.BC8.Idx47 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGTACCTGrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGTACCTG rGrGrG
TSO.id48 B.TSO.N10.BC8.Idx48 ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNNAGCGTCCTrGrGrG ACACTCTTTCCCTACACGACG/ideoxyU//ideoxyU/NNNNNNNNNN AGCGTCCT rGrGrG
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG
  • The 3' adaptor list
Tube ID Primer full name Primer full sequence PE_R Barcode T20VN
T20.r01 T20VN_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT \ TTTTTTTTTTTTTTTTTTTTVN
T20.r02 N9_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTNNNNNNNNN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT \ NNNNNNNNN
T20.r03 T20VN_BC6_PE_R GCATTCCTGCTGAACCGCTCTTTTTAGGTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTT TTTAGG TTTTTTTTTTTTTTTTTTTTVN
T20.id01 T20VN.BC8.PE_R.Indx1 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCTGAGAC TTTTTTTTTTTTTTTTTTTTVN
T20.id02 T20VN.BC8.PE_R.Indx2 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TGCCCAAG TTTTTTTTTTTTTTTTTTTTVN
T20.id03 T20VN.BC8.PE_R.Indx3 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GACTCGCT TTTTTTTTTTTTTTTTTTTTVN
T20.id04 T20VN.BC8.PE_R.Indx4 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CCACTCCA TTTTTTTTTTTTTTTTTTTTVN
T20.id05 T20VN.BC8.PE_R.Indx5 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GAACAGGG TTTTTTTTTTTTTTTTTTTTVN
T20.id06 T20VN.BC8.PE_R.Indx6 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTGCTGAT TTTTTTTTTTTTTTTTTTTTVN
T20.id07 T20VN.BC8.PE_R.Indx7 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTAGTATC TTTTTTTTTTTTTTTTTTTTVN
T20.id08 T20VN.BC8.PE_R.Indx8 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGCTGAAA TTTTTTTTTTTTTTTTTTTTVN
T20.id09 T20VN.BC8.PE_R.Indx9 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GAGTGCTT TTTTTTTTTTTTTTTTTTTTVN
T20.id10 T20VN.BC8.PE_R.Indx10 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT ATACGCCA TTTTTTTTTTTTTTTTTTTTVN
T20.id11 T20VN.BC8.PE_R.Indx11 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT ATGCGTTA TTTTTTTTTTTTTTTTTTTTVN
T20.id12 T20VN.BC8.PE_R.Indx12 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCACCCTT TTTTTTTTTTTTTTTTTTTTVN
T20.id13 T20V.BC8.PE_R.Indx13 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CCAACCGC TTTTTTTTTTTTTTTTTTTTV
T20.id14 T20V.BC8.PE_R.Indx14 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGATTACC TTTTTTTTTTTTTTTTTTTTV
T20.id15 T20V.BC8.PE_R.Indx15 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAGAGACA TTTTTTTTTTTTTTTTTTTTV
T20.id16 T20V.BC8.PE_R.Indx16 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAATAGTG TTTTTTTTTTTTTTTTTTTTV
T20.id17 T20V.BC8.PE_R.Indx17 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGTGGCCA TTTTTTTTTTTTTTTTTTTTV
T20.id18 T20V.BC8.PE_R.Indx18 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GGAGGGAG TTTTTTTTTTTTTTTTTTTTV
T20.id19 T20V.BC8.PE_R.Indx19 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GTTGTCCT TTTTTTTTTTTTTTTTTTTTV
T20.id20 T20V.BC8.PE_R.Indx20 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TTCGACCT TTTTTTTTTTTTTTTTTTTTV
T20.id21 T20V.BC8.PE_R.Indx21 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GCTTATTG TTTTTTTTTTTTTTTTTTTTV
T20.id22 T20V.BC8.PE_R.Indx22 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CAACCTTT TTTTTTTTTTTTTTTTTTTTV
T20.id23 T20V.BC8.PE_R.Indx23 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT CTTAGGTA TTTTTTTTTTTTTTTTTTTTV
T20.id24 T20V.BC8.PE_R.Indx24 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT GCTCACCA TTTTTTTTTTTTTTTTTTTTV
N2.id73 PCR_R.N2Ind73 CAAGCAGAAGACGGCATACGAGATGAGTCCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAGTCC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id74 PCR_R.N2Ind74 CAAGCAGAAGACGGCATACGAGATTCGCCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TCGCCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id75 PCR_R.N2Ind75 CAAGCAGAAGACGGCATACGAGATAAGTCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AAGTCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id76 PCR_R.N2Ind76 CAAGCAGAAGACGGCATACGAGATAATAGGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AATAGG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id77 PCR_R.N2Ind77 CAAGCAGAAGACGGCATACGAGATACCCGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCCGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id78 PCR_R.N2Ind78 CAAGCAGAAGACGGCATACGAGATAACACGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AACACG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id79 PCR_R.N2Ind79 CAAGCAGAAGACGGCATACGAGATGCTTGGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTTGG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id80 PCR_R.N2Ind80 CAAGCAGAAGACGGCATACGAGATTTACCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TTACCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id81 PCR_R.N2Ind81 CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCAGGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id82 PCR_R.N2Ind82 CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTTTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id83 PCR_R.N2Ind83 CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GACCAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id84 PCR_R.N2Ind84 CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACAAGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id85 PCR_R.N2Ind85 CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCGCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id86 PCR_R.N2Ind86 CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGGTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id87 PCR_R.N2Ind87 CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ATTCCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id88 PCR_R.N2Ind88 CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAATGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id89 PCR_R.N2Ind89 CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id90 PCR_R.N2Ind90 CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AGTGCT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id91 PCR_R.N2Ind91 CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CAGGGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id92 PCR_R.N2Ind92 CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CATGCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id93 PCR_R.N2Ind93 CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGCCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id94 PCR_R.N2Ind94 CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTATAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id95 PCR_R.N2Ind95 CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCGAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id96 PCR_R.N2Ind96 CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCTGC CTCGGCATTCCTGCTGAACCGCTCTT