Hosuk:Cell fixation in culture dish: Difference between revisions
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*For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C) | *For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C) | ||
# | #200uL of formalin (~39% formaldehyde)directly, NO aspirate media, incubate for 15min at 37C | ||
#Change media of fresh formalin+PBS ( | #Change media of fresh formalin+PBS (No wash!), incubate for 15min at 37C, | ||
#Wash with PBS, and add 70% Ethanol (2mL) | #Wash with PBS three times, and add 70% Ethanol (2mL) | ||
#Store at 4C | #Store at 4C |
Revision as of 20:57, 7 February 2013
- For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
- 200uL of formalin (~39% formaldehyde)directly, NO aspirate media, incubate for 15min at 37C
- Change media of fresh formalin+PBS (No wash!), incubate for 15min at 37C,
- Wash with PBS three times, and add 70% Ethanol (2mL)
- Store at 4C