Blue/2013-3-4: Difference between revisions

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Performed the following totoRNAseq protocols on 96-well huES9 sorted cells:
1. Mg based RNA fragmentation (Exp 02272013)
2. RNaseIII based RNA fragmentation (Exp 02282013)
After combining 96 wells and precipitation, pellets were very large and could not be fully resuspended (likely excess salt)
Centrifuged to remove undisolved precipitate for TSO reaction (kept pellet).
Results of PCR  using Smarter PCR Kit:
[[File:02282013_PCR1_Pur_1.jpg]]
[[File:02282013_PCR1_Pur_1.jpg]]

Revision as of 00:30, 6 March 2013

Performed the following totoRNAseq protocols on 96-well huES9 sorted cells: 1. Mg based RNA fragmentation (Exp 02272013) 2. RNaseIII based RNA fragmentation (Exp 02282013)

After combining 96 wells and precipitation, pellets were very large and could not be fully resuspended (likely excess salt)

Centrifuged to remove undisolved precipitate for TSO reaction (kept pellet).

Results of PCR using Smarter PCR Kit:


File:02282013 PCR1 Pur 1.jpg