Blue/2013-3-4: Difference between revisions
Jump to navigation
Jump to search
>B1lake No edit summary |
>B1lake No edit summary |
||
Line 1: | Line 1: | ||
Performed the following totoRNAseq protocols on 96-well huES9 sorted cells: | |||
1. Mg based RNA fragmentation (Exp 02272013) | |||
2. RNaseIII based RNA fragmentation (Exp 02282013) | |||
After combining 96 wells and precipitation, pellets were very large and could not be fully resuspended (likely excess salt) | |||
Centrifuged to remove undisolved precipitate for TSO reaction (kept pellet). | |||
Results of PCR using Smarter PCR Kit: | |||
[[File:02282013_PCR1_Pur_1.jpg]] | [[File:02282013_PCR1_Pur_1.jpg]] |
Revision as of 00:30, 6 March 2013
Performed the following totoRNAseq protocols on 96-well huES9 sorted cells: 1. Mg based RNA fragmentation (Exp 02272013) 2. RNaseIII based RNA fragmentation (Exp 02282013)
After combining 96 wells and precipitation, pellets were very large and could not be fully resuspended (likely excess salt)
Centrifuged to remove undisolved precipitate for TSO reaction (kept pellet).
Results of PCR using Smarter PCR Kit: