Matthew Cai/LabNotes/2013-3-12: Difference between revisions

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(Created page with "===cDNA + RNase continued=== *Used RNase A from Thermo Scientific *Added 20ul H20 to each cDNA sample to bring total volume to 100ul *Added 1.5ul RNase A (10ug/ul) *Incubated ...")
 
>Mzcai
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===Probe Capture===
===Probe Capture===
*
*[[http://genome-tech.ucsd.edu/LabNotes/index.php?title=Noi/NOTES/2013-3-12|Continued on Noi's Notes]]

Revision as of 20:56, 6 April 2013

cDNA + RNase continued

  • Used RNase A from Thermo Scientific
  • Added 20ul H20 to each cDNA sample to bring total volume to 100ul
  • Added 1.5ul RNase A (10ug/ul)
  • Incubated for 15 min at 37C
  • Purified cDNA with Qiagen minelute column
  • Qubit conc:

Probe Capture