Matthew Cai/LabNotes/2013-3-12: Difference between revisions
Jump to navigation
Jump to search
>Mzcai (Created page with "===cDNA + RNase continued=== *Used RNase A from Thermo Scientific *Added 20ul H20 to each cDNA sample to bring total volume to 100ul *Added 1.5ul RNase A (10ug/ul) *Incubated ...") |
>Mzcai m (→Probe Capture) |
||
Line 8: | Line 8: | ||
===Probe Capture=== | ===Probe Capture=== | ||
* | *[[http://genome-tech.ucsd.edu/LabNotes/index.php?title=Noi/NOTES/2013-3-12|Continued on Noi's Notes]] |
Revision as of 20:56, 6 April 2013
cDNA + RNase continued
- Used RNase A from Thermo Scientific
- Added 20ul H20 to each cDNA sample to bring total volume to 100ul
- Added 1.5ul RNase A (10ug/ul)
- Incubated for 15 min at 37C
- Purified cDNA with Qiagen minelute column
- Qubit conc: