EricChu:LabNotesMDA/2013-3-25: Difference between revisions
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* 4-5ul of samples were collected from each chamber. | * 4-5ul of samples were collected from each chamber. | ||
[[File:03252013qPCR.jpg]] |
Revision as of 19:38, 26 March 2013
Using MEF and High Concentration Phi29 in the Vertical Chamber Device
- pH adjustment for device was tested prior to this experiment. pH of ALS+NS should be = 6.0 to get a neutral pH in the device.
- 2 cells were captured in the capture chamber. Cells were lysed in the device.
- Air pushing lysed sample to the next area. But visible amount of samples remained in the mixing rings.
- Mixing MDA mix and cell contents on device. No observable DNA.
- After 5 hours 30C on TE module, clusters of DNA were visible in the high vertical chambers but not the rounded channels. However, the number of clusters (visible DNA) were lower in this experiment.
- 4-5ul of samples were collected from each chamber.