Daniel:Notebook/HiResChrPaint/2013-4-15: Difference between revisions

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   Two samples chosen from [[Daniel:Notebook/HiResChrPaint/2013-4-10|Gel Quantification (4/10/13)]].  
   Two samples chosen from [[Daniel:Notebook/HiResChrPaint/2013-4-10|Gel Quantification (4/10/13)]].  
   Oligos coupled to appropriate dye.
   Oligos coupled to appropriate dye.
===Dye Coupling Reaction===
  Thaw out oligos, sodium bicarbonate solution, DMSO
    Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
  Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
  Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
  Add 1 uL of appropriate dye to oligo set (see below)


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]

Revision as of 18:14, 15 April 2013

Dye Coupling Test and Gel Quantification

 Purpose:  Test dye coupling reaction, specifically the purification step, to ensure proper dye-coupled DNA is being captured.
 Two samples chosen from Gel Quantification (4/10/13). 
 Oligos coupled to appropriate dye.

Dye Coupling Reaction

 Thaw out oligos, sodium bicarbonate solution, DMSO
    Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
 Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
 Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
 Add 1 uL of appropriate dye to oligo set (see below)

Back to Calendar