Daniel:Notebook/HiResChrPaint/2013-4-15: Difference between revisions
Jump to navigation
Jump to search
>Djacobse No edit summary |
>Djacobse No edit summary |
||
Line 3: | Line 3: | ||
Two samples chosen from [[Daniel:Notebook/HiResChrPaint/2013-4-10|Gel Quantification (4/10/13)]]. | Two samples chosen from [[Daniel:Notebook/HiResChrPaint/2013-4-10|Gel Quantification (4/10/13)]]. | ||
Oligos coupled to appropriate dye. | Oligos coupled to appropriate dye. | ||
===Dye Coupling Reaction=== | |||
Thaw out oligos, sodium bicarbonate solution, DMSO | |||
Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C | |||
Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf | |||
Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO | |||
Add 1 uL of appropriate dye to oligo set (see below) | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Revision as of 18:14, 15 April 2013
Dye Coupling Test and Gel Quantification
Purpose: Test dye coupling reaction, specifically the purification step, to ensure proper dye-coupled DNA is being captured. Two samples chosen from Gel Quantification (4/10/13). Oligos coupled to appropriate dye.
Dye Coupling Reaction
Thaw out oligos, sodium bicarbonate solution, DMSO Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO Add 1 uL of appropriate dye to oligo set (see below)