Daniel:Notebook/HiResChrPaint/2013-4-15: Difference between revisions
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Use dyes immediately, they do not store | Use dyes immediately, they do not store | ||
4. Add 1 uL of appropriate dye to oligo set (see below) | 4. Add 1 uL of appropriate dye to oligo set (see below) | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt" align="center" | |||
| width="65" height="16" | Probe | |||
| width="65" | Alexa Dye | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V4S1 | |||
| align="right" | 488 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V4S2 | |||
| align="right" | 546 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V4S3 | |||
| align="right" | 594 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V4S4 | |||
| align="right" | 647 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V6S1 | |||
| align="right" | 488 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V6S2 | |||
| align="right" | 546 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V6S3 | |||
| align="right" | 594 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | V6S4 | |||
| align="right" | 647 | |||
|} | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Revision as of 18:17, 15 April 2013
Dye Coupling Test and Gel Quantification
Purpose: Test dye coupling reaction, specifically the purification step, to ensure proper dye-coupled DNA is being captured. Two samples chosen from Gel Quantification (4/10/13). Oligos coupled to appropriate dye.
Dye Coupling Reaction
1. Thaw out oligos, sodium bicarbonate solution, DMSO Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C 2. Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf 3. Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO Use dyes immediately, they do not store 4. Add 1 uL of appropriate dye to oligo set (see below)
Probe | Alexa Dye |
V4S1 | 488 |
V4S2 | 546 |
V4S3 | 594 |
V4S4 | 647 |
V6S1 | 488 |
V6S2 | 546 |
V6S3 | 594 |
V6S4 | 647 |