Daniel:Notebook/HiResChrPaint/2013-3-26: Difference between revisions
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==Expansion PCR and Ethanol Precipitation== | ==Expansion PCR and Ethanol Precipitation== | ||
=== | ===Expansion PCR=== | ||
1. Set up 8 reactions for each of the 8 probes (64 reactions total) | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt" align="center" | |||
|style="background-color:#FFFF00" align="right" width="149" height="16" | | |||
|style="font-weight:bold" width="65" | per rxn | |||
|style="font-weight:bold" width="65" | V4S1 | |||
|style="font-weight:bold" width="65" | V4S2 | |||
|style="font-weight:bold" width="65" | V4S3 | |||
|style="font-weight:bold" width="65" | V4S4 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 20 nM OligoPool | |||
| align="right" | 0.2 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 2X Kapa SYBR qPCR MM | |||
| align="right" | 50 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 100 uM AP1V41U | |||
| align="right" | 0.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 100 uM AP2V4 | |||
| align="right" | 0.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 2 mM aa-dUTP | |||
| align="right" | 3 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | H20 | |||
| align="right" | 46 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | Total Volume | |||
| align="right" | 100 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
|- style="font-size:12pt" align="center" | |||
|style="background-color:#FFFF00" align="right" height="16" | | |||
|style="font-weight:bold" | per rxn | |||
|style="font-weight:bold" | V6S1 | |||
|style="font-weight:bold" | V6S2 | |||
|style="font-weight:bold" | V6S3 | |||
|style="font-weight:bold" | V6S6 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 20 nM OligoPool | |||
| align="right" | 0.2 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
| align="right" | 1.7 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 2X Kapa SYBR qPCR MM | |||
| align="right" | 50 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
| align="right" | 425 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 100 uM AP1V61U | |||
| align="right" | 0.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 100 uM AP2V6 | |||
| align="right" | 0.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
| align="right" | 3.4 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | 2 mM aa-dUTP | |||
| align="right" | 3 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
| align="right" | 25.5 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | H20 | |||
| align="right" | 46 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
| align="right" | 391 | |||
|- style="font-size:12pt" align="center" | |||
| height="16" | Total Volume | |||
| align="right" | 100 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
| align="right" | 850 | |||
|} |
Revision as of 22:51, 17 April 2013
Probe Set Prep (Started 3/26/2013)
Expansion PCR and Ethanol Precipitation
Expansion PCR
1. Set up 8 reactions for each of the 8 probes (64 reactions total)
per rxn | V4S1 | V4S2 | V4S3 | V4S4 | |
20 nM OligoPool | 0.2 | 1.7 | 1.7 | 1.7 | 1.7 |
2X Kapa SYBR qPCR MM | 50 | 425 | 425 | 425 | 425 |
100 uM AP1V41U | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
100 uM AP2V4 | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
2 mM aa-dUTP | 3 | 25.5 | 25.5 | 25.5 | 25.5 |
H20 | 46 | 391 | 391 | 391 | 391 |
Total Volume | 100 | 850 | 850 | 850 | 850 |
per rxn | V6S1 | V6S2 | V6S3 | V6S6 | |
20 nM OligoPool | 0.2 | 1.7 | 1.7 | 1.7 | 1.7 |
2X Kapa SYBR qPCR MM | 50 | 425 | 425 | 425 | 425 |
100 uM AP1V61U | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
100 uM AP2V6 | 0.4 | 3.4 | 3.4 | 3.4 | 3.4 |
2 mM aa-dUTP | 3 | 25.5 | 25.5 | 25.5 | 25.5 |
H20 | 46 | 391 | 391 | 391 | 391 |
Total Volume | 100 | 850 | 850 | 850 | 850 |