Daniel:Notebook/HiResChrPaint/2013-3-27: Difference between revisions
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>Djacobse (Created page with "=Probe Set Prep (Started 3/26/2013)= Back to Calendar ==Expansion PCR and Ethanol Precipitation==") |
>Djacobse No edit summary |
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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
== | ==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-3-26|From 3/26/2013]])== | ||
4. Centrifuge at 3000 rpm for 30 min, 4 C | |||
5. Remove Supernatant and add 600 uL chilled 75% EtOH | |||
6. Transfer DNA pellet to a 1.5 mL tube | |||
7. Spin at 14000 rpm for 5 min | |||
8. Dry the pellet in the hood and resuspend with 100 uL ddH2O | |||
9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column |
Revision as of 23:08, 17 April 2013
Probe Set Prep (Started 3/26/2013)
Ethanol Precipitation (From 3/26/2013)
4. Centrifuge at 3000 rpm for 30 min, 4 C 5. Remove Supernatant and add 600 uL chilled 75% EtOH 6. Transfer DNA pellet to a 1.5 mL tube 7. Spin at 14000 rpm for 5 min 8. Dry the pellet in the hood and resuspend with 100 uL ddH2O 9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column