Daniel:Notebook/HiResChrPaint/2013-4-19: Difference between revisions

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(Created page with "=Probe Prep Synthesis (Started 3/26/13)= ==TBU Gel (from EtOH Precipitation 4/18/2013)=...")
 
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   9. Imaged in gel doc
   9. Imaged in gel doc
==Gel Images==
==Gel Images==
[[Image:ProbeQuant_EtOH_041913.jpg|600px]]




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Revision as of 20:36, 19 April 2013

Probe Prep Synthesis (Started 3/26/13)

TBU Gel (from EtOH Precipitation 4/18/2013)

 1. Preran gel for ~45 minutes at 250V
 2. Ran 2 gels w/5 lanes each (out of 12 well gels)
 3. 5.5 uL clean TBE, 0.5 uL sample, 6 uL 2X Urea buffer (0.5 ladder also)
 4. Precooked samples at 65C for 8 minutes
 5. Loaded all 12 uL into each lane
 6. Ran gels for 20 minutes at 250 V
 7. Stained with 3 uL SYBR gold for 30 minutes
 8. Rinsed, let sit for ~20 minutes 
     *this isn't recommended but I didn't have time to image immediately
 9. Imaged in gel doc

Gel Images

File:ProbeQuant EtOH 041913.jpg


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