Daniel:Notebook/HiResChrPaint/2013-4-19: Difference between revisions
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>Djacobse (Created page with "=Probe Prep Synthesis (Started 3/26/13)= ==TBU Gel (from EtOH Precipitation 4/18/2013)=...") |
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9. Imaged in gel doc | 9. Imaged in gel doc | ||
==Gel Images== | ==Gel Images== | ||
[[Image:ProbeQuant_EtOH_041913.jpg|600px]] | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Revision as of 20:36, 19 April 2013
Probe Prep Synthesis (Started 3/26/13)
TBU Gel (from EtOH Precipitation 4/18/2013)
1. Preran gel for ~45 minutes at 250V 2. Ran 2 gels w/5 lanes each (out of 12 well gels) 3. 5.5 uL clean TBE, 0.5 uL sample, 6 uL 2X Urea buffer (0.5 ladder also) 4. Precooked samples at 65C for 8 minutes 5. Loaded all 12 uL into each lane 6. Ran gels for 20 minutes at 250 V 7. Stained with 3 uL SYBR gold for 30 minutes 8. Rinsed, let sit for ~20 minutes *this isn't recommended but I didn't have time to image immediately 9. Imaged in gel doc