Daniel:Notebook/HiResChrPaint/2013-4-25: Difference between revisions

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[[Daniel:Notebook/HiResChrPaint|Back to calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to calendar]]
==DpnII Digest (Continued from [[Daniel:Notebook/HiResChrPaint/2013-4-24|4/24/2013]])==
1. Took samples from thermocycler
2. Purified using [[Daniel:Protocols/Qiagen_Column|Qiagen column]]
3. Set up following TBE gel
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| width="65" height="36" | Sample
|style="background-color:#D9D9D9" width="65" | Ladder
|style="background-color:#B7DEE8" width="65" | V4S3A-Pre Digestion
|style="background-color:#B7DEE8" width="65" | V4S3B-Pre Digestion
|style="background-color:#FABF8F" width="65" | V4S3A-Post Digestion
|style="background-color:#CCC0DA" width="65" | V4S3A-Post Column
|style="background-color:#CCC0DA" width="65" | V4S3B-Post Column
|style="background-color:#D9D9D9" width="65" | Ladder
|style="background-color:#B7DEE8" width="65" | V6S3A-Pre Digestion
|style="background-color:#B7DEE8" width="65" | V6S3B-Pre Digestion
|style="background-color:#FABF8F" width="65" | V6S3A-Post Digestion
|style="background-color:#CCC0DA" width="65" | V6S3A-Post Column
|style="background-color:#CCC0DA" width="65" | V6S3B-Post Column
|- style="font-size:12pt" align="center"
| height="15" | uL sample
|style="background-color:#D9D9D9" align="center" | 1
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#FABF8F" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#D9D9D9" align="center" | 1
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#FABF8F" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|- style="font-size:12pt" align="center"
| height="15" | uL buffer
|style="background-color:#D9D9D9" align="center" | 5
|style="background-color:#B7DEE8" align="center" | 4
|style="background-color:#B7DEE8" align="center" | 4
|style="background-color:#FABF8F" align="center" | 4
|style="background-color:#CCC0DA" align="center" | 4
|style="background-color:#CCC0DA" align="center" | 4
|style="background-color:#D9D9D9" align="center" | 5
|style="background-color:#B7DEE8" align="center" | 4
|style="background-color:#B7DEE8" align="center" | 4
|style="background-color:#FABF8F" align="center" | 4
|style="background-color:#CCC0DA" align="center" | 4
|style="background-color:#CCC0DA" align="center" | 4
|- style="font-size:12pt" align="center"
| height="15" | uL dye
|style="background-color:#D9D9D9" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#FABF8F" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#D9D9D9" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#B7DEE8" align="center" | 2
|style="background-color:#FABF8F" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|style="background-color:#CCC0DA" align="center" | 2
|- style="font-size:12pt" align="center"
| height="15" | loaded
|style="background-color:#D9D9D9" align="center" | 7
|style="background-color:#B7DEE8" align="center" | 7
|style="background-color:#B7DEE8" align="center" | 7
|style="background-color:#FABF8F" align="center" | 7
|style="background-color:#CCC0DA" align="center" | 7
|style="background-color:#CCC0DA" align="center" | 7
|style="background-color:#D9D9D9" align="center" | 7
|style="background-color:#B7DEE8" align="center" | 7
|style="background-color:#B7DEE8" align="center" | 7
|style="background-color:#FABF8F" align="center" | 7
|style="background-color:#CCC0DA" align="center" | 7
|style="background-color:#CCC0DA" align="center" | 7
|}
4. Ran gel for 30 minutes at 200 V.
5. Stained for 10 minutes with 3 uL SYBR gold
6. Measured concentration in Nanodrop
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| width="65" height="15" | Sample
| width="65" | ng/uL
| width="65" | uL
| width="65" | ng
|- style="font-size:12pt" align="center"
| height="15" | V4S3A
| align="center" | 102.2
| align="center" | 30
| align="center" | 3066
|- style="font-size:12pt" align="center"
| height="15" | V4S3B
| align="center" | 86.8
| align="center" | 30
| align="center" | 2604
|- style="font-size:12pt" align="center"
| height="15" | V6S3A
| align="center" | 80.1
| align="center" | 30
| align="center" | 2403
|- style="font-size:12pt" align="center"
| height="15" | V6S3B
| align="center" | 77.9
| align="center" | 30
| align="center" | 2337
|}
===Gel Results===


[[Image:DpnII_Digest_042513.jpg|600px]]
[[Image:DpnII_Digest_042513.jpg|600px]]

Revision as of 21:19, 25 April 2013

Probe Prep Test (Started 4/22/2013)

Back to calendar

DpnII Digest (Continued from 4/24/2013)

1. Took samples from thermocycler
2. Purified using Qiagen column
3. Set up following TBE gel
Sample Ladder V4S3A-Pre Digestion V4S3B-Pre Digestion V4S3A-Post Digestion V4S3A-Post Column V4S3B-Post Column Ladder V6S3A-Pre Digestion V6S3B-Pre Digestion V6S3A-Post Digestion V6S3A-Post Column V6S3B-Post Column
uL sample 1 2 2 2 2 2 1 2 2 2 2 2
uL buffer 5 4 4 4 4 4 5 4 4 4 4 4
uL dye 2 2 2 2 2 2 2 2 2 2 2 2
loaded 7 7 7 7 7 7 7 7 7 7 7 7
4. Ran gel for 30 minutes at 200 V.
5. Stained for 10 minutes with 3 uL SYBR gold
6. Measured concentration in Nanodrop

Nanodrop Results

Sample ng/uL uL ng
V4S3A 102.2 30 3066
V4S3B 86.8 30 2604
V6S3A 80.1 30 2403
V6S3B 77.9 30 2337

Gel Results

File:DpnII Digest 042513.jpg