Daniel:Notebook/HiResChrPaint/2013-4-25: Difference between revisions
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1. Took samples from thermocycler | 1. Took samples from thermocycler | ||
2. Purified using [[Daniel:Protocols/Qiaquick_Column|Qiagen column]] | 2. Purified using [[Daniel:Protocols/Qiaquick_Column|Qiagen column]], elute with 30 uL | ||
3. Set up following TBE gel | 3. Set up following TBE gel | ||
Revision as of 21:20, 25 April 2013
Probe Prep Test (Started 4/22/2013)
DpnII Digest (Continued from 4/24/2013)
1. Took samples from thermocycler 2. Purified using Qiagen column, elute with 30 uL 3. Set up following TBE gel
Sample | Ladder | V4S3A-Pre Digestion | V4S3B-Pre Digestion | V4S3A-Post Digestion | V4S3A-Post Column | V4S3B-Post Column | Ladder | V6S3A-Pre Digestion | V6S3B-Pre Digestion | V6S3A-Post Digestion | V6S3A-Post Column | V6S3B-Post Column |
uL sample | 1 | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 2 |
uL buffer | 5 | 4 | 4 | 4 | 4 | 4 | 5 | 4 | 4 | 4 | 4 | 4 |
uL dye | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
loaded | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
4. Ran gel for 30 minutes at 200 V. 5. Stained for 10 minutes with 3 uL SYBR gold 6. Measured concentration in Nanodrop
Nanodrop Results
Sample | ng/uL | uL | ng |
V4S3A | 102.2 | 30 | 3066 |
V4S3B | 86.8 | 30 | 2604 |
V6S3A | 80.1 | 30 | 2403 |
V6S3B | 77.9 | 30 | 2337 |