Daniel:Notebook/HiResChrPaint/2013-4-25: Difference between revisions

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  1. Took samples from thermocycler
  1. Took samples from thermocycler
  2. Purified using [[Daniel:Protocols/Qiaquick_Column|Qiagen column]]
  2. Purified using [[Daniel:Protocols/Qiaquick_Column|Qiagen column]], elute with 30 uL
  3. Set up following TBE gel
  3. Set up following TBE gel



Revision as of 21:20, 25 April 2013

Probe Prep Test (Started 4/22/2013)

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DpnII Digest (Continued from 4/24/2013)

1. Took samples from thermocycler
2. Purified using Qiagen column, elute with 30 uL
3. Set up following TBE gel
Sample Ladder V4S3A-Pre Digestion V4S3B-Pre Digestion V4S3A-Post Digestion V4S3A-Post Column V4S3B-Post Column Ladder V6S3A-Pre Digestion V6S3B-Pre Digestion V6S3A-Post Digestion V6S3A-Post Column V6S3B-Post Column
uL sample 1 2 2 2 2 2 1 2 2 2 2 2
uL buffer 5 4 4 4 4 4 5 4 4 4 4 4
uL dye 2 2 2 2 2 2 2 2 2 2 2 2
loaded 7 7 7 7 7 7 7 7 7 7 7 7
4. Ran gel for 30 minutes at 200 V.
5. Stained for 10 minutes with 3 uL SYBR gold
6. Measured concentration in Nanodrop

Nanodrop Results

Sample ng/uL uL ng
V4S3A 102.2 30 3066
V4S3B 86.8 30 2604
V6S3A 80.1 30 2403
V6S3B 77.9 30 2337

Gel Results

File:DpnII Digest 042513.jpg