Daniel:Reagents/LambdaExonuclease: Difference between revisions
Jump to navigation
Jump to search
>Djacobse No edit summary |
>Djacobse No edit summary |
||
Line 3: | Line 3: | ||
Lambda Exonuclease datasheet (NEB): [[File:M0262-lambda-exonuclease.pdf]] | Lambda Exonuclease datasheet (NEB): [[File:M0262-lambda-exonuclease.pdf]] | ||
*1 unit is defined as the amount of | *1 unit is defined as the amount of enzyme required to produce 10 nmol of deoxyribonucleotide from double-stranded | ||
substrate in a total reaction volume of 50 uL in 30 minutes at 37C in 1X reaction buffer with 1 ug of H3-DNA (NEB) | |||
Heat Shock | |||
Heat inactivate for 10 minutes at 75 C. | |||
NEB | NEB |
Latest revision as of 16:36, 3 May 2013
Lambda Exonuclease[edit]
Lambda Exonuclease datasheet (NEB): File:M0262-lambda-exonuclease.pdf
*1 unit is defined as the amount of enzyme required to produce 10 nmol of deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 uL in 30 minutes at 37C in 1X reaction buffer with 1 ug of H3-DNA (NEB)
Heat Shock
Heat inactivate for 10 minutes at 75 C.
NEB
M0262S 1,000 units 5,000 units/ml $63.00 M0262L 5,000 units 5,000 units/ml $252.00