Daniel:Notebook/HiResChrPaint/2013-4-27: Difference between revisions
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Volume Report: [[File:GelQuant_042713.xls]] | Volume Report: [[File:GelQuant_042713.xls]] | ||
[[Image:GelQuant_042713.jpg|600px]] | |||
[[Image:GelQuant_analysis_042713.jpg|600px]] |
Revision as of 00:51, 28 April 2013
Probe Prep Test (Started 4/22/2013)
Ethanol Precipitation (Continued from 4/26/2013)
5. Took samples from -80 and centrifuged at 14000 rpm for 25 minutes at 4 C 6. Removed supernatant and added 750 uL 75% EtOH 7. Centrifugd at 14000 rpm for 15 minutes at 4 C 8. Removed most supernatant with pipette and then dried in hood 9. Resuspended in 8 uL nuclease free water
TBU gel Quantification
1. Preheated TBE buffer in 60 C incubator 2. microwaved for 1 minute 3. Added hot TBE to gel box and immediately preran gel for 20 minutes at 250V 4. Created samples according to following table
Lane | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
Sample | Ladder | Ladder | V4S3 | V4S3 | V6S3 | V6S3 | Ladder | Buffer |
uL sample | 0.5 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0 |
uL hot TBE | 5.5 | 5 | 5.5 | 5.5 | 5.5 | 5.5 | 5.5 | 6 |
uL 2x buffer | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
loaded | 12 | 12 | 12 | 12 | 12 | 12 | 12 | 12 |
5. Incubated samples at 65C for 8 minutes 6. Cold snapped with ice box 7. Loaded 12 uL each sample and ran gel for 20 minutes at 250V 8. Stained with 3 uL SYBR gold for 25 minutes
Gel Results
Volume Report: File:GelQuant 042713.xls