Daniel:Notebook/HiResChrPaint/2013-4-29: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Probe Prep Test (Started 4/22/2013)= Back to calendar =TBE Gel= Purpose: As per [[Daniel:Not...")
 
>Djacobse
No edit summary
Line 32: Line 32:
|}
|}


  2. Ran gel for 20 minutes at 200 V
  2. Ran gel for 25 minutes at 200 V
  3. Stained with 3 uL SYBR gold for 5 min
  3. Stained with 3 uL SYBR gold for 5 min

Revision as of 21:54, 29 April 2013

Probe Prep Test (Started 4/22/2013)

Back to calendar

TBE Gel

Purpose: As per 4/27/2013 results, I need to better understand the 
 lambda exonuclease digestion.  To this end I'm going to play around with the parameters of the reaction.  To do
 so I need several samples to work with.  I am testing/identifying samples right now that will suit my purposes.

Protocol

1. Prepared samples (1 uL sample, 5 uL TBE, 2 uL 6x dye) and loaded (7 uL) according to table
Lane 2 3 4 5 6
Sample Ladder V4S2 V6S2 V6S3 V6S3 Digest
2. Ran gel for 25 minutes at 200 V
3. Stained with 3 uL SYBR gold for 5 min