Hosuk:LabNotes/2013-5-2: Difference between revisions

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====Result - RT check====
====Result - RT check====
*based on Debugging plan ([[Hosuk:LabNotes/2013-5-1|05/01]])
*based on Debugging plan ([[Hosuk:LabNotes/2013-5-1|05/01]])


*RT process seemed work.
*RT process seemed work.

Revision as of 22:35, 2 May 2013

Result - RT check

  • based on Debugging plan (05/01)


  • RT process seemed work.
  • +RTase sample showed very strong Cy5 signal which is supposed cDNA
  • comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
  • Most of signal came from cell structure.
  • However I don't know what are the large spots. Are they also cDNAs?
  • -RTase sample has very low intensity of signals in the cell, and
  • not many spots were observed, which spots were outside of cells.
  • +RTase
S2:+RTase, Brighfield, 20x S2:+RTase, Cy5, 20x S2:+RTase, Merged
(Original) (Original)
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite +RTase fig07-08.jpg


  • -RTase (No RTase)
S3:-RTase, Brighfield, 20x S3:-RTase, Cy5, 20x S3:-RTase, Merged
(Original) (Original)
File:RTCheckNoRTase fig08 20xobj pos05 BF exp0.2-EM00.jpg File:RTCheckNoRTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite NoRTase fig07-08.jpg