Matt:LabNotes/2013-5-16: Difference between revisions
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>Mzcai (Created page with "Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-15 ===Column Purification=== *Purified 6 tubes with 12 columns (100ul each) Nanodrop conc:") |
>Mzcai |
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===Column Purification=== | ===Column Purification=== | ||
*Purified 6 tubes with 12 columns (100ul each) | *Purified 6 tubes of 200ul enzyme digested probes with 12 columns (100ul in each column) | ||
*Eluted 30ul each (180ul total per probe set) | |||
Nanodrop conc: | Nanodrop ssDNA conc: <br> | ||
0 gap: 58.78 ng/ul * 180ul = 10.58ug <br> | |||
20 gap: 55.96 ng/ul * 180ul = 10.07ug <br> |
Revision as of 22:19, 16 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-15
Column Purification
- Purified 6 tubes of 200ul enzyme digested probes with 12 columns (100ul in each column)
- Eluted 30ul each (180ul total per probe set)
Nanodrop ssDNA conc:
0 gap: 58.78 ng/ul * 180ul = 10.58ug
20 gap: 55.96 ng/ul * 180ul = 10.07ug