Daniel:Protocols/AlexaDyeCouple: Difference between revisions

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Alexa Protocol: [[File:ARESTM DNA Labeling Kits.pdf]]
Alexa Protocol: [[File:ARESTM DNA Labeling Kits.pdf]]
==General Protocol==
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
    *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
8. Recommend column purification after incubation

Revision as of 16:55, 20 May 2013

Alexa Fluorophore Dye Coupling

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Alexa Protocol: File:ARESTM DNA Labeling Kits.pdf

General Protocol

1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
8. Recommend column purification after incubation