Daniel:Protocols/AlexaDyeCouple: Difference between revisions

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  7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
  7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
  8. Recommend column purification after incubation
  8. Recommend column purification after incubation
===Dye Couples===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#FFFF00;font-weight:bold" align="center"
| width="69" height="12" | Probe
| width="69" | Alexa Dye
|-  align="center"
| height="12" | V4S1
| Alexa-546
|-  align="center"
| height="12" | V4S2
| Alexa-488
|-  align="center"
| height="12" | V4S3
| Alexa-594
|-  align="center"
| height="12" | V4S4
| Alexa-647
|-  align="center"
| height="12" | V6S1
| Alexa-546
|-  align="center"
| height="12" | V6S2
| Alexa-488
|-  align="center"
| height="12" | V6S3
| Alexa-594
|-  align="center"
| height="12" | V6S4
| Alexa-647
|}

Revision as of 16:57, 20 May 2013

Alexa Fluorophore Dye Coupling

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Alexa Protocol: File:ARESTM DNA Labeling Kits.pdf

General Protocol

1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
8. Recommend column purification after incubation

Dye Couples

Probe Alexa Dye
V4S1 Alexa-546
V4S2 Alexa-488
V4S3 Alexa-594
V4S4 Alexa-647
V6S1 Alexa-546
V6S2 Alexa-488
V6S3 Alexa-594
V6S4 Alexa-647