Daniel:Protocols/AlexaDyeCouple: Difference between revisions

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==ULYSIS Protocol===
==ULYSIS Protocol===


ULYSIS Manual from Invitrogen: [[ULYSIS_Manual.pdf]]
ULYSIS Manual from Invitrogen: [[File:ULYSIS_Manual.pdf]]


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Revision as of 18:48, 3 July 2013

Alexa Fluorophore Dye Coupling

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Dye Couples

Probe Alexa Dye
V4S1 Alexa-546
V4S2 Alexa-488
V4S3 Alexa-594
V4S4 Alexa-647
V6S1 Alexa-546
V6S2 Alexa-488
V6S3 Alexa-594
V6S4 Alexa-647

ARES Protocol

ARES Manual from Invitrogen: File:ARESTM DNA Labeling Kits.pdf

1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
8. Recommend column purification after incubation

ULYSIS Protocol=

ULYSIS Manual from Invitrogen: File:ULYSIS Manual.pdf

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