Matt:LabNotes/2013-5-21: Difference between revisions
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*Load wells with 2, 4, 6, 8 ul | *Load wells with 2, 4, 6, 8 ul | ||
Continued on: | Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22 |
Latest revision as of 19:30, 22 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-17
Ethanol Precipitation after PAGE size selection[edit]
- Spun 6 tubes in -80C at 10,000rpm at 4C for 30min
- Discard supernatant and add 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min
- Discard supernatant and let dry in hood 10 min
- Resuspended each of the 6 tubes with 10ul H2O (60ul total)
TBU Gel Quantification Plan[edit]
- Dilute 2ul into 20ul
- Mix 1:1 with 2X buffer (20X dilution)
- Load wells with 2, 4, 6, 8 ul
Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22