Blue:RNA-Seq Experiments:05172013: Difference between revisions
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'''Purpose:''' Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr | '''Purpose:''' Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr | ||
'''Outcome:''' Optimal range between 58C and 65C, drop in efficiency above 65C. | '''Outcome:''' Optimal range between 58C and 65C, drop in efficiency above 65C. Also poor amplification if only the first stage of the PCR protocol is used. | ||
[[File:05172013.pdf]] | [[File:05172013.pdf]] |
Latest revision as of 00:17, 22 May 2013
Library: UHRR from 05162013
Purpose: Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr
Outcome: Optimal range between 58C and 65C, drop in efficiency above 65C. Also poor amplification if only the first stage of the PCR protocol is used.