Blue:RNA-Seq Experiments:05172013: Difference between revisions

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'''Purpose:''' Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr  
'''Purpose:''' Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr  


'''Outcome:''' Optimal range between 58C and 65C, drop in efficiency above 65C.
'''Outcome:''' Optimal range between 58C and 65C, drop in efficiency above 65C.  Also poor amplification if only the first stage of the PCR protocol is used.


[[File:05172013.pdf]]
[[File:05172013.pdf]]

Latest revision as of 00:17, 22 May 2013

Library: UHRR from 05162013

Purpose: Test for optimal annealing temperature for PB_PCR_F/PGR_PCR_R using Sybr

Outcome: Optimal range between 58C and 65C, drop in efficiency above 65C. Also poor amplification if only the first stage of the PCR protocol is used.

File:05172013.pdf