Daniel:Notebook/HiResChrPaint/2013-6-4: Difference between revisions
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>Djacobse (Created page with "=Probe Prep 3 (Started 5/29/2013)= Back to Calendar ==DpnII Digest (from [[Daniel:Notebook/HiRe...") |
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==DpnII Digest (from [[Daniel:Notebook/HiResChrPaint|6/3/2013]])== | ==DpnII Digest (from [[Daniel:Notebook/HiResChrPaint|6/3/2013]])== | ||
4. [[Daniel: | 4. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], elute with 30 uL | ||
5. Measure concentrations in Nanodrop | 5. Measure concentrations in Nanodrop | ||
Revision as of 21:28, 4 June 2013
Probe Prep 3 (Started 5/29/2013)
DpnII Digest (from 6/3/2013)
4. Column Purification, elute with 30 uL 5. Measure concentrations in Nanodrop
Nanodrop Results
Sample | ng/uL | ug in 30 uL | Yield (%) |
alpha-4B | 127.1 | 3.8 | 80.9 |
alpha-6B | 122.4 | 3.7 | 78.6 |
beta-4B | 122.6 | 3.7 | 83.6 |
beta-6B | 112.5 | 3.4 | 73.3 |
TBE Gel
1. Gel setup
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 10 bp ladder | V4S2-B** | V4S2-A | V6S2-A | V4S2-A* | V4S2-B* | 10 bp ladder | V6S2-B** | V4S2-A | V6S2-A | V4S2-A* | V4S2-B* |
2. Ran gel for 25 minutes at 250 V 3. Stained with 3 uL SYBR gold for 3 minutes