Daniel:Notebook/HiResChrPaint/2013-6-4: Difference between revisions

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==DpnII Digest (from [[Daniel:Notebook/HiResChrPaint|6/3/2013]])==
==DpnII Digest (from [[Daniel:Notebook/HiResChrPaint|6/3/2013]])==


  4. [[Daniel:Protocol/Qiaquick_Column|Column Purification]], elute with 30 uL
  4. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], elute with 30 uL
  5. Measure concentrations in Nanodrop
  5. Measure concentrations in Nanodrop



Revision as of 21:28, 4 June 2013

Probe Prep 3 (Started 5/29/2013)

Back to Calendar

DpnII Digest (from 6/3/2013)

4. Column Purification, elute with 30 uL
5. Measure concentrations in Nanodrop

Nanodrop Results

Sample ng/uL ug in 30 uL Yield (%)
alpha-4B 127.1 3.8 80.9
alpha-6B 122.4 3.7 78.6
beta-4B 122.6 3.7 83.6
beta-6B 112.5 3.4 73.3

TBE Gel

1. Gel setup
Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 10 bp ladder V4S2-B** V4S2-A V6S2-A V4S2-A* V4S2-B* 10 bp ladder V6S2-B** V4S2-A V6S2-A V4S2-A* V4S2-B*
2. Ran gel for 25 minutes at 250 V
3. Stained with 3 uL SYBR gold for 3 minutes

Gel Image