Daniel:Notebook/HiResChrPaint/2013-6-5: Difference between revisions

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>Djacobse
(Created page with "=Probe Prep 3 (Started 5/29/2013)= Back to Calendar ==DpnII Digestion== Finishing the digest ...")
 
>Djacobse
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  1. Set up  
  1. Set up  


C group
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom"
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom"
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|- style="font-size:12pt" align="center" valign="bottom"
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | alpha 4-B
| height="15" | alpha 4-C
| align="center" | 35
| align="center" | 35
| align="center" | 2
| align="center" | 2
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|- style="font-size:12pt" align="center" valign="bottom"
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | alpha 6-B
| height="15" | alpha 6-C
| align="center" | 35
| align="center" | 35
| align="center" | 2
| align="center" | 2
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|- style="font-size:12pt" align="center" valign="bottom"
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | beta 4-B
| height="15" | beta 4-C
| align="center" | 35
| align="center" | 35
| align="center" | 2
| align="center" | 2
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|- style="font-size:12pt" align="center" valign="bottom"
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | beta 6-B
| height="15" | beta 6-C
| align="center" | 35
| align="center" | 2
| align="center" | 5
| align="center" | 8
| align="center" | 50
 
|}
 
D Group
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom"
| width="65" height="30" | Sample
| width="65" | uL sample
| width="65" | uL Dpn II (50 U/uL)
| width="65" | uL 10x buffer
| width="65" | uL H20
| width="65" | total
 
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | alpha 4-D
| align="center" | 35
| align="center" | 2
| align="center" | 5
| align="center" | 8
| align="center" | 50
 
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | alpha 6-D
| align="center" | 35
| align="center" | 2
| align="center" | 5
| align="center" | 8
| align="center" | 50
 
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | beta 4-D
| align="center" | 35
| align="center" | 2
| align="center" | 5
| align="center" | 8
| align="center" | 50
 
|- style="font-size:12pt" align="center" valign="bottom"
| height="15" | beta 6-D
| align="center" | 35
| align="center" | 35
| align="center" | 2
| align="center" | 2

Revision as of 22:41, 5 June 2013

Probe Prep 3 (Started 5/29/2013)

Back to Calendar

DpnII Digestion

Finishing the digest on the PCR amplified probes, aa-dUTP and no aa-dUTP.  Will run for 2 hours since I didn't see any qualitative difference
between the two.
1. Set up 

C group

Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-C 35 2 5 8 50
alpha 6-C 35 2 5 8 50
beta 4-C 35 2 5 8 50
beta 6-C 35 2 5 8 50

D Group

Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-D 35 2 5 8 50
alpha 6-D 35 2 5 8 50
beta 4-D 35 2 5 8 50
beta 6-D 35 2 5 8 50
2. Incubate in thermocycler at 37C for 16 hours; heat shock 20 minutes at 65 C