Daniel:Notebook/HiResChrPaint/2013-6-5: Difference between revisions
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>Djacobse (Created page with "=Probe Prep 3 (Started 5/29/2013)= Back to Calendar ==DpnII Digestion== Finishing the digest ...") |
>Djacobse No edit summary |
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1. Set up | 1. Set up | ||
C group | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom" | |- style="font-size:12pt;font-weight:bold" align="center" valign="bottom" | ||
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|- style="font-size:12pt" align="center" valign="bottom" | |- style="font-size:12pt" align="center" valign="bottom" | ||
| height="15" | alpha 4- | | height="15" | alpha 4-C | ||
| align="center" | 35 | | align="center" | 35 | ||
| align="center" | 2 | | align="center" | 2 | ||
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|- style="font-size:12pt" align="center" valign="bottom" | |- style="font-size:12pt" align="center" valign="bottom" | ||
| height="15" | alpha 6- | | height="15" | alpha 6-C | ||
| align="center" | 35 | | align="center" | 35 | ||
| align="center" | 2 | | align="center" | 2 | ||
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|- style="font-size:12pt" align="center" valign="bottom" | |- style="font-size:12pt" align="center" valign="bottom" | ||
| height="15" | beta 4- | | height="15" | beta 4-C | ||
| align="center" | 35 | | align="center" | 35 | ||
| align="center" | 2 | | align="center" | 2 | ||
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|- style="font-size:12pt" align="center" valign="bottom" | |- style="font-size:12pt" align="center" valign="bottom" | ||
| height="15" | beta 6- | | height="15" | beta 6-C | ||
| align="center" | 35 | |||
| align="center" | 2 | |||
| align="center" | 5 | |||
| align="center" | 8 | |||
| align="center" | 50 | |||
|} | |||
D Group | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom" | |||
| width="65" height="30" | Sample | |||
| width="65" | uL sample | |||
| width="65" | uL Dpn II (50 U/uL) | |||
| width="65" | uL 10x buffer | |||
| width="65" | uL H20 | |||
| width="65" | total | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
| height="15" | alpha 4-D | |||
| align="center" | 35 | |||
| align="center" | 2 | |||
| align="center" | 5 | |||
| align="center" | 8 | |||
| align="center" | 50 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
| height="15" | alpha 6-D | |||
| align="center" | 35 | |||
| align="center" | 2 | |||
| align="center" | 5 | |||
| align="center" | 8 | |||
| align="center" | 50 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
| height="15" | beta 4-D | |||
| align="center" | 35 | |||
| align="center" | 2 | |||
| align="center" | 5 | |||
| align="center" | 8 | |||
| align="center" | 50 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
| height="15" | beta 6-D | |||
| align="center" | 35 | | align="center" | 35 | ||
| align="center" | 2 | | align="center" | 2 |
Revision as of 22:41, 5 June 2013
Probe Prep 3 (Started 5/29/2013)
DpnII Digestion
Finishing the digest on the PCR amplified probes, aa-dUTP and no aa-dUTP. Will run for 2 hours since I didn't see any qualitative difference between the two.
1. Set up
C group
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-C | 35 | 2 | 5 | 8 | 50 |
alpha 6-C | 35 | 2 | 5 | 8 | 50 |
beta 4-C | 35 | 2 | 5 | 8 | 50 |
beta 6-C | 35 | 2 | 5 | 8 | 50 |
D Group
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-D | 35 | 2 | 5 | 8 | 50 |
alpha 6-D | 35 | 2 | 5 | 8 | 50 |
beta 4-D | 35 | 2 | 5 | 8 | 50 |
beta 6-D | 35 | 2 | 5 | 8 | 50 |
2. Incubate in thermocycler at 37C for 16 hours; heat shock 20 minutes at 65 C