Hosuk:LabNotes/2013-6-10: Difference between revisions

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>Hosuki78
(Created page with "=====Training Day 1===== *Some of important things *Phi29 enzyme from '''Enzymatics''' is used. *Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are f...")
 
>Hosuki78
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*RNase H step after RT is critical step, so do this carefully.
*RNase H step after RT is critical step, so do this carefully.
*Try HeLa cells unless I get rolonies with PGP1F.
*Try HeLa cells unless I get rolonies with PGP1F.
*The sample (that I've got some signal at 05/29) doesn't seem to have actual rolonies,
*There are no dots, just too much even signal distribution.
*

Revision as of 19:50, 10 June 2013

Training Day 1
  • Some of important things
  • Phi29 enzyme from Enzymatics is used.
  • Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are from Enzymatics.
  • RNase H step after RT is critical step, so do this carefully.
  • Try HeLa cells unless I get rolonies with PGP1F.


  • The sample (that I've got some signal at 05/29) doesn't seem to have actual rolonies,
  • There are no dots, just too much even signal distribution.