Hosuk:LabNotes/2013-6-10: Difference between revisions
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>Hosuki78 (Created page with "=====Training Day 1===== *Some of important things *Phi29 enzyme from '''Enzymatics''' is used. *Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are f...") |
>Hosuki78 No edit summary |
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*RNase H step after RT is critical step, so do this carefully. | *RNase H step after RT is critical step, so do this carefully. | ||
*Try HeLa cells unless I get rolonies with PGP1F. | *Try HeLa cells unless I get rolonies with PGP1F. | ||
*The sample (that I've got some signal at 05/29) doesn't seem to have actual rolonies, | |||
*There are no dots, just too much even signal distribution. | |||
* |
Revision as of 19:50, 10 June 2013
Training Day 1
- Some of important things
- Phi29 enzyme from Enzymatics is used.
- Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are from Enzymatics.
- RNase H step after RT is critical step, so do this carefully.
- Try HeLa cells unless I get rolonies with PGP1F.
- The sample (that I've got some signal at 05/29) doesn't seem to have actual rolonies,
- There are no dots, just too much even signal distribution.