Daniel:Notebook/HiResChrPaint/2013-6-10: Difference between revisions

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=Probe Prep 3 ([[Daniel:Notebook/HiResChrPaint/2013-5-29|Started 5/29/2013]])=


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
==TBU Gel Quantification==
[[Daniel:Protocols/TBUGel|Protocol 2]]
1. Sample matrix
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" width="65" height="15" | Lane
| align="center" width="65" | 2
| align="center" width="65" | 3
| align="center" width="65" | 4
| align="center" width="65" | 5
| align="center" width="65" | 6
| align="center" width="65" | 7
| align="center" width="65" | 8
| align="center" width="65" | 9
| align="center" width="65" | 10
| align="center" width="65" | 11
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom"
| height="30" | Sample
| Low Mass Ladder
| Low Mass Ladder
| V4S2
| V6S2
| V4S2*
| V6S2*
| V4S2
| V6S2
| V4S2*
| V6S2*
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | uL sample
| align="center" | 0.5
| align="center" | 1
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.5
| 2**
| 2**
| 2**
| 2**
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | uL TBE
| align="center" | 1.5
| align="center" | 1
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 9.5
| align="center" | 1
| align="center" | 1
| align="center" | 1
| align="center" | 1
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="30" | uL 2X Urea Buffer
| align="center" | 2
| align="center" | 2
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 10
| align="center" | 1
| align="center" | 1
| align="center" | 1
| align="center" | 1
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | Dilution
| NA
| NA
| align="center" | 1:10
| align="center" | 1:10
| align="center" | 1:10
| align="center" | 1:10
| align="center" | 1:20
| align="center" | 1:20
| align="center" | 1:20
| align="center" | 1:20


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | Loaded
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
| align="center" | 4
 
|}
 
2. Ran gel for 18 minutes at 250 V
3. Stained for 25 minutes with 3 uL SYBR Gold
 
===Results===


Volume Report: [[File:GelQuant_061013.xls]]
Volume Report: [[File:GelQuant_061013.xls]]

Revision as of 00:45, 11 June 2013

Probe Prep 3 (Started 5/29/2013)

Back to Calendar

TBU Gel Quantification

Protocol 2

1. Sample matrix
Lane 2 3 4 5 6 7 8 9 10 11
Sample Low Mass Ladder Low Mass Ladder V4S2 V6S2 V4S2* V6S2* V4S2 V6S2 V4S2* V6S2*
uL sample 0.5 1 0.5 0.5 0.5 0.5 2** 2** 2** 2**
uL TBE 1.5 1 9.5 9.5 9.5 9.5 1 1 1 1
uL 2X Urea Buffer 2 2 10 10 10 10 1 1 1 1
Dilution NA NA 1:10 1:10 1:10 1:10 1:20 1:20 1:20 1:20
Loaded 4 4 4 4 4 4 4 4 4 4
2. Ran gel for 18 minutes at 250 V
3. Stained for 25 minutes with 3 uL SYBR Gold

Results

Volume Report: File:GelQuant 061013.xls

File:GelQuant 061013.jpg

File:GelQuant 051513 Volume.jpg