Matt:LabNotes/2013-6-12: Difference between revisions

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===Verify Probe Capture===


Primers: <br>
CP-2-FA - 'GCACGATCCGACGGTAGTGT'<br>
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br>
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''Volume (ul)'''
|-
| Circle Oligos||5
|-
| 10uM CP-2-FA||1
|-
| 10uM CP-2-RA||1
|-
| 2X KAPA SYBG||25
|-
| H2O||18
|-
| Total||50
|-
|
|}
  Program
  98C 30sec--> (98C 10sec --> 55C 30sec --> 72C 30sec)x25 cycles --> 72C 3 min --> 15C hold

Latest revision as of 22:37, 13 June 2013