Hosuk:LabNotesMDA/2013-6-21: Difference between revisions

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*qPCR result was not correct because of using wrong primers --> re-run
*qPCR result was not correct because of using wrong primers --> re-run
**For the initial test, 7 samples - 2, 5, 8, 11, 13, 18, 20, 22 were run.
**For the initial test, 7 samples - 2, 5, 8, 11, 13, 18, 20, 22 were run.
{| {{table}}
| align="center" |10ng
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| align="center" |10ng
|
| align="center" |1ng
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| align="center" |1ng
|
| align="center" |100pg
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| align="center" |100pg
|
| align="center" |10pg
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| align="center" |10pg
|-
| align="center" |NTC
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| align="center" |#2
|
| align="center" |#5
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| align="center" |#11
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| align="center" |#13
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| align="center" |#18
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| align="center" |#20
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| align="center" |#22
|-
|
|}




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{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber #'''
| align="center" style="background:#f0f0f0;"|'''Chamber #'''
||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume (uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Mass (pg/uL)'''
| align="center" style="background:#f0f0f0;"|'''Mass (pg/uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Total (pg)'''
| align="center" style="background:#f0f0f0;"|'''Total (pg)'''
||
|-
|-
|1 ||5.05 | || | ||  
|1  
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|align="center" |5.05  
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|  
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|2 ||5.42 | || | ||  
|2  
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|align="center" |5.42  
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|3 ||5.36 | || | ||  
|3  
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|align="center" |5.36  
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|4 ||5.08 | || | ||  
|4  
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|align="center" |5.08  
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|5 ||6.87 | || | ||  
|5  
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|align="center" |6.87  
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|6 ||5.46 | || | ||  
|6  
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|align="center" |5.46  
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|7 ||5.42 | || | ||  
|7  
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|align="center" |5.42  
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|8 ||5.24 | || | ||  
|8  
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|align="center" |5.24
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|9 ||5.22 | || | ||  
|9  
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|align="center" |5.22
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|10 ||4.80 | || | ||  
|10  
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|align="center" |4.80  
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|11 ||5.34 | || | ||  
|11  
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|align="center" |5.34
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|12 ||5.15 | || | ||  
|12  
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|align="center" |5.15  
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|13 ||4.25 | || | ||  
|13  
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|align="center" |4.25  
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|14 ||5.01 | || | ||  
|14  
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|align="center" |5.01
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|  
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|15 ||4.83 | || | ||  
|15  
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|align="center" |4.83  
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|  
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|16 ||4.58 | || | ||  
|16  
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|align="center" |4.58
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|17 ||5.93 | || | ||  
|17  
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|align="center" |5.93
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|  
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|18 ||5.74 | || | ||  
|18  
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|align="center" |5.74
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|19 ||5.19 | || | ||  
|19  
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|align="center" |5.19
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|20 ||5.40 | || | ||  
|20  
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|align="center" |5.40  
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|21 ||6.02 | || | ||  
|21  
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|align="center" |6.02  
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|22 ||4.93 | || | ||  
|22  
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|align="center" |4.93
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|23 ||5.43 | || | ||  
|23  
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|align="center" |5.43  
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|24 ||4.60 | || | ||  
|24
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|align="center" |4.60
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|}
|}

Revision as of 18:15, 21 June 2013

MDA with PGP1F, 4th - qPCR

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run
    • For the initial test, 7 samples - 2, 5, 8, 11, 13, 18, 20, 22 were run.
10ng 10ng 1ng 1ng 100pg 100pg 10pg 10pg
NTC #2 #5 #11 #13 #18 #20 #22


Samples
Chamber # Volume (uL) Mass (pg/uL) Total (pg)
1 5.05
2 5.42
3 5.36
4 5.08
5 6.87
6 5.46
7 5.42
8 5.24
9 5.22
10 4.80
11 5.34
12 5.15
13 4.25
14 5.01
15 4.83
16 4.58
17 5.93
18 5.74
19 5.19
20 5.40
21 6.02
22 4.93
23 5.43
24 4.60