Hosuk:LabNotesMDA/2013-6-28: Difference between revisions

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>Hosuki78
Line 5: Line 5:
*'''400mM ALS''' --> original protocol
*'''400mM ALS''' --> original protocol
*'''Slow mixing''' --> 1 sec period, mixing for 5min
*'''Slow mixing''' --> 1 sec period, mixing for 5min
*'''Run MDA in ring mixer''' --> 25th chamber!
*Push ring mixer volume with air
*Push ring mixer volume with air
**Left 8 chamber were empty, too many empty chamber...
**Left 8 chamber were empty, too many empty chamber...
Line 12: Line 13:
====Verification with qPCR====
====Verification with qPCR====
*Result looked good.
*Result looked good.
*Most of chambers have 500pg~2400pg of amplicons.
*Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.


*[[File:qPCR_Result_2013-06-28_Edited.png|800px]]
*[[File:qPCR_Result_2013-06-28_Edited.png|800px]]

Revision as of 17:47, 28 June 2013

MDA with PGP1F, 6th

  • MDA run for 8hr @06/27
  • 400mM ALS --> original protocol
  • Slow mixing --> 1 sec period, mixing for 5min
  • Run MDA in ring mixer --> 25th chamber!
  • Push ring mixer volume with air
    • Left 8 chamber were empty, too many empty chamber...


Verification with qPCR

  • Result looked good.
  • Most of chambers have 500pg~2400pg of amplicons.
  • Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.


CoRE fragmentation

  • Done @06/28