Hosuk:LabNotesMDA/2013-6-28: Difference between revisions
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>Hosuki78 |
>Hosuki78 |
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*'''400mM ALS''' --> original protocol | *'''400mM ALS''' --> original protocol | ||
*'''Slow mixing''' --> 1 sec period, mixing for 5min | *'''Slow mixing''' --> 1 sec period, mixing for 5min | ||
*'''Run MDA in ring mixer''' --> 25th chamber! | |||
*Push ring mixer volume with air | *Push ring mixer volume with air | ||
**Left 8 chamber were empty, too many empty chamber... | **Left 8 chamber were empty, too many empty chamber... | ||
Line 12: | Line 13: | ||
====Verification with qPCR==== | ====Verification with qPCR==== | ||
*Result looked good. | *Result looked good. | ||
*Most of chambers have 500pg~2400pg of amplicons. | |||
*Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected. | |||
*[[File:qPCR_Result_2013-06-28_Edited.png|800px]] | *[[File:qPCR_Result_2013-06-28_Edited.png|800px]] |
Revision as of 17:47, 28 June 2013
MDA with PGP1F, 6th
- MDA run for 8hr @06/27
- 400mM ALS --> original protocol
- Slow mixing --> 1 sec period, mixing for 5min
- Run MDA in ring mixer --> 25th chamber!
- Push ring mixer volume with air
- Left 8 chamber were empty, too many empty chamber...
Verification with qPCR
- Result looked good.
- Most of chambers have 500pg~2400pg of amplicons.
- Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.
CoRE fragmentation
- Done @06/28