Hosuk:LabNotesMDA/2013-6-28: Difference between revisions
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>Hosuki78 (→Result) |
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*Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected. | *Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected. | ||
*25th chamber(Ring mixer) had less amount than other chamber did, but there was amplicon! | *25th chamber(Ring mixer) had less amount than other chamber did, but there was amplicon! | ||
*It seemed that low conc. of ALS doesn’t work in the MDA device… I’ll try higher conc. | *It seemed that low conc. of ALS doesn’t work in the MDA device… I’ll try higher conc. than 133mM, such as 250mM ALS. | ||
Revision as of 17:51, 28 June 2013
MDA with PGP1F, 6th
- MDA run for 8hr @06/27
- 400mM ALS --> original protocol
- Slow mixing --> 1 sec period, mixing for 5min
- Run MDA in ring mixer --> 25th chamber!
- Push ring mixer volume with air
- Left 8 chamber were empty, too many empty chamber...
Result
- qPCR result looked good.
- Most of chambers have 500pg~2400pg of amplicons.
- Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.
- 25th chamber(Ring mixer) had less amount than other chamber did, but there was amplicon!
- It seemed that low conc. of ALS doesn’t work in the MDA device… I’ll try higher conc. than 133mM, such as 250mM ALS.
File:QPCR Result 2013-06-28 Edited.png File:QPCR Result MassTable 2013-06-28.png
CoRE fragmentation
- Done @06/28