Blue:RNAseq Primer List: Difference between revisions

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| TSO.r04||iso_B_TSO_N10||iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG
| TSO.r04||iso_B_TSO_N10||iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG
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| TSO.r05||IsoL_B_TSO_N8||T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G
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| PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||||||
| PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||||||
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Revision as of 16:16, 7 July 2013


Adaptor Structure

Rui's totoRNAseq Page


totoRNA primer sequence

  • The 5' adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r04 iso_B_TSO_N10 iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r05 IsoL_B_TSO_N8 T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G \ rGrG+G
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG