Blue:RNAseq Primer List: Difference between revisions

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>B1lake
>B1lake
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* The 3' adaptors list
* The 3' adaptors list
{| {{table}} border=1
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''Tube ID'''
| align="center" style="background:#f0f0f0;"|'''Primer full name'''
| align="center" style="background:#f0f0f0;"|'''Primer full name'''
| align="center" style="background:#f0f0f0;"|'''Primer full sequence'''
| align="center" style="background:#f0f0f0;"|'''Primer full sequence'''
| align="center" style="background:#f0f0f0;"|'''T20 Complementary Seq'''
| align="center" style="background:#f0f0f0;"|'''T20 Complementary Seq'''
| align="center" style="background:#f0f0f0;"|'''Novel T7 Seq'''
| align="center" style="background:#f0f0f0;"|'''Novel T7 Seq'''
| align="center" style="background:#f0f0f0;"|''''''
|-
|-
| RL.Add.T7||||CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA||GCATTCCTGCTGAACCGCTCTT||CTTTAATTATGCTGAGTGATATCCC||
| RL.Add.T7||CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA||GCATTCCTGCTGAACCGCTCTT||CTTTAATTATGCTGAGTGATATCCC
|-
|-
|}
|}

Revision as of 21:15, 15 July 2013


Adaptor Structure

Rui's totoRNAseq Page


totoRNA 5' primer sequence

  • The 5' TSO adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r04 iso_B_TSO_N10 iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r05 IsoL_B_TSO_N8 T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G \ rGrG+G
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
PB_PCR.F* PB_PCR.Fmod AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
ILMN_PCR.F* ILMN_PCR.Fmod AATGATACGGCGACCACCGAGATC A*A*T*GATACGGCGACCACCGAGATC \


totoRNA 3' primer sequence

  • The 3' T20 adaptors list
Tube ID Primer full name Primer full sequence PE_R Barcode T20V
T20V.id02 T20V.BC8.PE_R.Indx2 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TGCCCAAG TTTTTTTTTTTTTTTTTTTTV
T20V.id03 T20V.BC8.PE_R.Indx3 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV GACTCGCT
T20V.id04 T20V.BC8.PE_R.Indx4 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV CCACTCCA
T20V.id05 T20V.BC8.PE_R.Indx5 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV GAACAGGG
T20V.id06 T20V.BC8.PE_R.Indx6 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV CTGCTGAT
T20V.id07 T20V.BC8.PE_R.Indx7 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV CTAGTATC
T20V.id08 T20V.BC8.PE_R.Indx8 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV GGCTGAAA
T20V.id09 T20V.BC8.PE_R.Indx9 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV GAGTGCTT
T20V.id10 T20V.BC8.PE_R.Indx10 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV ATACGCCA
T20V.id11 T20V.BC8.PE_R.Indx11 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV ATGCGTTA
T20V.id12 T20V.BC8.PE_R.Indx12 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV TCACCCTT
T20V.id13 T20V.BC8.PE_R.Indx13 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV CCAACCGC
T20V.id14 T20V.BC8.PE_R.Indx14 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV GGATTACC
T20V.id15 T20V.BC8.PE_R.Indx15 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV CAGAGACA
T20V.id16 T20V.BC8.PE_R.Indx16 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV CAATAGTG
T20V.id17 T20V.BC8.PE_R.Indx17 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV GGTGGCCA
T20V.id18 T20V.BC8.PE_R.Indx18 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV GGAGGGAG
T20V.id19 T20V.BC8.PE_R.Indx19 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV GTTGTCCT
T20V.id20 T20V.BC8.PE_R.Indx20 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV TTCGACC
T20V.id21 T20V.BC8.PE_R.Indx21 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV GCTTATTG
T20V.id22 T20V.BC8.PE_R.Indx22 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV CAACCTTT
T20V.id23 T20V.BC8.PE_R.Indx23 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV CTTAGGTA
T20V.id24 T20V.BC8.PE_R.Indx24 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV GCTCACCA
T20V.id25 T20V.BC8.PE_R.Indx25 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV AGAAATGC
T20V.id26 T20V.BC8.PE_R.Indx26 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV TACTGATT
T20V.id27 T20V.BC8.PE_R.Indx27 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV CTCACGGG
T20V.id28 T20V.BC8.PE_R.Indx28 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV
T20V.id29 T20V.BC8.PE_R.Indx29 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV
T20V.id30 T20V.BC8.PE_R.Indx30 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV
T20V.id31 T20V.BC8.PE_R.Indx31 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV
T20V.id32 T20V.BC8.PE_R.Indx32 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id33 T20V.BC8.PE_R.Indx33 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id34 T20V.BC8.PE_R.Indx34 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV
T20V.id35 T20V.BC8.PE_R.Indx35 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV
T20V.id36 T20V.BC8.PE_R.Indx36 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV
T20V.id37 T20V.BC8.PE_R.Indx37 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV
T20V.id38 T20V.BC8.PE_R.Indx38 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id39 T20V.BC8.PE_R.Indx39 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id40 T20V.BC8.PE_R.Indx40 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV
T20V.id41 T20V.BC8.PE_R.Indx41 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV
T20V.id42 T20V.BC8.PE_R.Indx42 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV
T20V.id43 T20V.BC8.PE_R.Indx43 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV
T20V.id44 T20V.BC8.PE_R.Indx44 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id45 T20V.BC8.PE_R.Indx45 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id46 T20V.BC8.PE_R.Indx46 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id47 T20V.BC8.PE_R.Indx47 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV
T20V.id48 T20V.BC8.PE_R.Indx48 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id49 T20V.BC8.PE_R.Indx49 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV
T20V.id50 T20V.BC8.PE_R.Indx50 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV
T20V.id51 T20V.BC8.PE_R.Indx51 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id52 T20V.BC8.PE_R.Indx52 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV
T20V.id53 T20V.BC8.PE_R.Indx53 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id54 T20V.BC8.PE_R.Indx54 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV
T20V.id55 T20V.BC8.PE_R.Indx55 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV
T20V.id56 T20V.BC8.PE_R.Indx56 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV
T20V.id57 T20V.BC8.PE_R.Indx57 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id58 T20V.BC8.PE_R.Indx58 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id59 T20V.BC8.PE_R.Indx59 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV
T20V.id60 T20V.BC8.PE_R.Indx60 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id61 T20V.BC8.PE_R.Indx61 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV
T20V.id62 T20V.BC8.PE_R.Indx62 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV
T20V.id63 T20V.BC8.PE_R.Indx63 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id64 T20V.BC8.PE_R.Indx64 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id65 T20V.BC8.PE_R.Indx65 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id66 T20V.BC8.PE_R.Indx66 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV
T20V.id67 T20V.BC8.PE_R.Indx67 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV
T20V.id68 T20V.BC8.PE_R.Indx68 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV
T20V.id69 T20V.BC8.PE_R.Indx69 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV
T20V.id70 T20V.BC8.PE_R.Indx70 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id71 T20V.BC8.PE_R.Indx71 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id72 T20V.BC8.PE_R.Indx72 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id73 T20V.BC8.PE_R.Indx73 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id74 T20V.BC8.PE_R.Indx74 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id75 T20V.BC8.PE_R.Indx75 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV
T20V.id76 T20V.BC8.PE_R.Indx76 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV
T20V.id77 T20V.BC8.PE_R.Indx77 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV
T20V.id78 T20V.BC8.PE_R.Indx78 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV
T20V.id79 T20V.BC8.PE_R.Indx79 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV
T20V.id80 T20V.BC8.PE_R.Indx80 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV
T20V.id81 T20V.BC8.PE_R.Indx81 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV
T20V.id82 T20V.BC8.PE_R.Indx82 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id83 T20V.BC8.PE_R.Indx83 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id84 T20V.BC8.PE_R.Indx84 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV
T20V.id85 T20V.BC8.PE_R.Indx85 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV
T20V.id86 T20V.BC8.PE_R.Indx86 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV
T20V.id87 T20V.BC8.PE_R.Indx87 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id88 T20V.BC8.PE_R.Indx88 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV
T20V.id89 T20V.BC8.PE_R.Indx89 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV
T20V.id90 T20V.BC8.PE_R.Indx90 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV
T20V.id91 T20V.BC8.PE_R.Indx91 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV
T20V.id92 T20V.BC8.PE_R.Indx92 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id93 T20V.BC8.PE_R.Indx93 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV
T20V.id94 T20V.BC8.PE_R.Indx94 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV
T20V.id95 T20V.BC8.PE_R.Indx95 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id96 T20V.BC8.PE_R.Indx96 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV


IVT 3' primer sequence

  • The 3' adaptors list
Primer full name Primer full sequence T20 Complementary Seq Novel T7 Seq
RL.Add.T7 CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA GCATTCCTGCTGAACCGCTCTT CTTTAATTATGCTGAGTGATATCCC

Sequencing Primers

Primer full name Primer full sequence Protocol Read
totoRNAseq Read 1 v2 CCACCGAGATCTACACTCTTTCCCTACACGACG totoRNAseq Read1
N2RevSeq2 CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT totoRNAseq Read2
N2IndSeq AAGAGCGGTTCAGCAGGAATGCCGAG totoRNAseq N2 index Read