Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions
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===Results=== | ===Results=== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
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|style="font-weight:bold" height=" | |style="font-weight:bold" height="15" | dye/100bp | ||
| align="center" | 0. | | align="center" | 0.0 | ||
| align="center" | | | align="center" | 0.7 | ||
| align="center" | | | align="center" | 0.8 | ||
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Revision as of 21:16, 8 July 2013
S2 Probe Prep (Started 5/29/2013)
ULYSIS Dye Coupling (Test)
Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling. Going to try two for better confidence.
- Following ULYSIS protocol
1. Add 1 uL DNA in 19 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample; add 4 uL labeling buffer 4. Incubate for 15 minutes at 80C, snap cool on ice 5. Purify using Centri-Sep Column
Results
AmpR | AmpR 1 | AmpR 2 | |
ng/uL DNA | 538 | 58.1 | 51.6 |
pmol/uL dye | 0.1 | 1.2 | 1.2 |
ug DNA | 0.5 | 1.2 | 1.0 |
pmol/uL DNA | 32.6 | 3.5 | 3.1 |
dye/probe | 0.003 | 0.341 | 0.384 |
dye/100bp | 0.0 | 0.7 | 0.8 |