Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions

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===Results===
===Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt"
|- style="font-size:12pt"
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|- style="font-size:12pt" align="center" valign="bottom"
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="30" | labeling efficiency
|style="font-weight:bold" height="15" | dye/100bp
| align="center" | 0.2
| align="center" | 0.0
| align="center" | 17.0
| align="center" | 0.7
| align="center" | 19.2
| align="center" | 0.8


|}
|}

Revision as of 21:16, 8 July 2013

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ULYSIS Dye Coupling (Test)

Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling.  Going to try two for better confidence.
1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample; add 4 uL labeling buffer
4. Incubate for 15 minutes at 80C, snap cool on ice
5. Purify using Centri-Sep Column

Results

  AmpR AmpR 1 AmpR 2
ng/uL DNA 538 58.1 51.6
pmol/uL dye 0.1 1.2 1.2
ug DNA 0.5 1.2 1.0
pmol/uL DNA 32.6 3.5 3.1
dye/probe 0.003 0.341 0.384
dye/100bp 0.0 0.7 0.8