Matt:LabNotes/2013-7-11: Difference between revisions

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*[http://genome-tech.ucsd.edu/LabNotes/index.php/Illumina_GA/Oligo_info#Primers_for_LC_Sciences_library-free_protocol Primers for Agi26k]
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Illumina_GA/Oligo_info#Primers_for_LC_Sciences_library-free_protocol Primers for Agi26k]
*Primers for CES36k18bp: AmpF6.3Sol and AmpR6.3Ind48
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Illumina_GA/Oligo_info#Library_Free_Multiplexing_Primers Primers for CES36k18bp: AmpF6.3Sol and AmpR6.3Ind48]


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Revision as of 22:21, 11 July 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-7-9

Amplification with Sequencing Adapters

Tube # Sample Index#
1 NTC-Agi26k_20gap Indx7
2 gDNA-Agi26k_20gap Indx77
3 cDNA-Agi26k_20gap Indx78
4 gDNA-CES36k18bp Indx48


Components 1X Reaction 5X Reaction
Captured Template 1 0
10uM Forward + Indx 0.4 0
10uM Reverse 0.4 2.0
2X KAPA SYBG FAST MM 12.5 62.5
H2O 10.7 53.5
Total 25 125
 Program
 *98°C 30sec -> (98°C 10sec -> 52°C 30sec -> 72°C 30sec) x8 cycles -> (98°C 10sec -> 72°C 30sec) x16 cycles -> 72°C 3 min -> 15°C hold