Daniel:Notebook/HiResChrPaint/2013-8-5: Difference between revisions
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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
Researched this morning on new ways to improve the FISH protocol. First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure). From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail). | Researched this morning on new ways to improve the FISH protocol. First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure). From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail). | ||
[[Image:Fish-hybridization-temp-compare.png|600px|thumb]] | [[Image:Fish-hybridization-temp-compare.png|600px|thumb]] | ||
For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus. Second, also in the thumbnail, | |||
==Lambda Exonuclease Digestion== | ==Lambda Exonuclease Digestion== |
Revision as of 19:08, 5 August 2013
S1 Probe Preparation (Started 8/2/2013
Researched this morning on new ways to improve the FISH protocol. First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure). From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail).
For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus. Second, also in the thumbnail,