Daniel:Notebook/HiResChrPaint/2013-8-5: Difference between revisions

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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
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Researched this morning on new ways to improve the FISH protocol.  First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure).  From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail).  For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus.  Second, also in the thumbnail,
Researched this morning on new ways to improve the FISH protocol.  First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure).  From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail).   


[[Image:Fish-hybridization-temp-compare.png|600px|thumb]]
[[Image:Fish-hybridization-temp-compare.png|600px|thumb]]
For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus.  Second, also in the thumbnail,






==Lambda Exonuclease Digestion==
==Lambda Exonuclease Digestion==

Revision as of 19:08, 5 August 2013

S1 Probe Preparation (Started 8/2/2013

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Researched this morning on new ways to improve the FISH protocol. First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure). From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail).

For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus. Second, also in the thumbnail,



Lambda Exonuclease Digestion