Hosuk:LabNotesMDA/2013-8-14: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 No edit summary |
>Hosuki78 No edit summary |
||
Line 26: | Line 26: | ||
*[[File:qPCR_Result_MDAv9.1_2013-08-13_QTable.png|700px]] | *[[File:qPCR_Result_MDAv9.1_2013-08-13_QTable.png|700px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-08-13_fig2_History.png| | *[[File:qPCR_Result_MDAv9.1_2013-08-13_fig2_History.png|600px]] | ||
[[File:qPCR_Result_MDAv9.1_2013-08-13_fig3_History_Recent.png| | [[File:qPCR_Result_MDAv9.1_2013-08-13_fig3_History_Recent.png|600px]] | ||
Revision as of 17:34, 14 August 2013
MDA with PGP1F, 12th, Dev v9.1
- Use mineral oil in valve lines
- I expect...
- No any leaking of H2O into flow channel
- Reduce cross-contamination
- But the response of valves became slower … maybe oil is compressed when pushing?
Experiment
- MDA reaction for 6 hr.
- qPCR run for verification
- Extract just almost 2uL at each tube and run qPCR.
Result
- Result : Good
- Looked more even than previous results.
- Amount of amplicons are detected more along to the right side of chambers
- Maybe the templates moved more toward right chambers?
- Would the amplicons are generated more? if the reaction time is longer...
File:QPCR Result MDAv9.1 2013-08-13 fig3 History Recent.png
Next Plan
- Water in valves for cell capture and mineral oil for rest of valves
- Try do Tagmentation
- File:Water in valves for cell capture MDAv9.1 2013-08-13.png