Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions
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===Nanodrop Results=== | ===Nanodrop Results=== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="65" height="15" | Sample | |||
| width="65" | BSPP 1AB | |||
| width="65" | BSPP 1CD | |||
| width="65" | BSPP 2AB | |||
| width="65" | BSPP 2CD | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ng/uL | |||
| align="center" | 127.7 | |||
| align="center" | 130.6 | |||
| align="center" | 110.1 | |||
| align="center" | 109.3 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug in 30 uL | |||
| align="center" | 3.8 | |||
| align="center" | 3.9 | |||
| align="center" | 3.3 | |||
| align="center" | 3.3 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | total | |||
| align="center" | | |||
| align="center" | 7.7 | |||
| align="center" | | |||
| align="center" | 6.6 | |||
|} | |||
===TBE Gel=== | ===TBE Gel=== |
Revision as of 18:46, 4 September 2013
BSPP Control (Started 09/03/2013)
DpnII Digestion (Started 09/03/2013)
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
Nanodrop Results
Sample | BSPP 1AB | BSPP 1CD | BSPP 2AB | BSPP 2CD |
ng/uL | 127.7 | 130.6 | 110.1 | 109.3 |
ug in 30 uL | 3.8 | 3.9 | 3.3 | 3.3 |
total | 7.7 | 6.6 |
TBE Gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp ladder | BSPP 1CD | BSPP 2AB | BSPP 2CD |
2. Run for 25 minutes at 200V 3. Stain with 3 uL SYBR gold for 2 minutes