Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions

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===Nanodrop Results===
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" height="15" | Sample
| width="65" | BSPP 1AB
| width="65" | BSPP 1CD
| width="65" | BSPP 2AB
| width="65" | BSPP 2CD
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 127.7
| align="center" | 130.6
| align="center" | 110.1
| align="center" | 109.3
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 30 uL
| align="center" | 3.8
| align="center" | 3.9
| align="center" | 3.3
| align="center" | 3.3
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | total
| align="center" | &nbsp;
| align="center" | 7.7
| align="center" | &nbsp;
| align="center" | 6.6
|}


===TBE Gel===
===TBE Gel===

Revision as of 18:46, 4 September 2013

BSPP Control (Started 09/03/2013)

Back to Calendar

DpnII Digestion (Started 09/03/2013)

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

Sample BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
ng/uL 127.7 130.6 110.1 109.3
ug in 30 uL 3.8 3.9 3.3 3.3
total   7.7   6.6

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 25 minutes at 200V
3. Stain with 3 uL SYBR gold for 2 minutes

Lambda Exonuclease Digestion