Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions
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{{GelLanes|Lane4=25bp ladder|Lane5=BSPP 1AB|Lane5=BSPP 1CD|Lane6=BSPP 2AB|Lane7=BSPP 2CD}} | {{GelLanes|Lane4=25bp ladder|Lane5=BSPP 1AB|Lane5=BSPP 1CD|Lane6=BSPP 2AB|Lane7=BSPP 2CD}} | ||
2. Run for | 2. Run for 30 minutes at 200V | ||
3. Stain with 3 uL SYBR gold for 2 minutes | 3. Stain with 3 uL SYBR gold for 2 minutes | ||
'''Gel Image''' | |||
==Lambda Exonuclease Digestion== | ==Lambda Exonuclease Digestion== |
Revision as of 18:46, 4 September 2013
BSPP Control (Started 09/03/2013)
DpnII Digestion (Started 09/03/2013)
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
Nanodrop Results
Sample | BSPP 1AB | BSPP 1CD | BSPP 2AB | BSPP 2CD |
ng/uL | 127.7 | 130.6 | 110.1 | 109.3 |
ug in 30 uL | 3.8 | 3.9 | 3.3 | 3.3 |
total | 7.7 | 6.6 |
TBE Gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp ladder | BSPP 1CD | BSPP 2AB | BSPP 2CD |
2. Run for 30 minutes at 200V 3. Stain with 3 uL SYBR gold for 2 minutes
Gel Image