Daniel:Notebook/HiResChrPaint/2013-9-4: Difference between revisions

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'''Gel Image'''
'''Gel Image'''
[[Image:2013-09-04-DpnIIDigest-BSPP.jpg|600px]]


==Lambda Exonuclease Digestion==
==Lambda Exonuclease Digestion==

Revision as of 19:00, 4 September 2013

BSPP Control (Started 09/03/2013)

Back to Calendar

DpnII Digestion (Started 09/03/2013)

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

Nanodrop Results

Sample BSPP 1AB BSPP 1CD BSPP 2AB BSPP 2CD
ng/uL 127.7 130.6 110.1 109.3
ug in 30 uL 3.8 3.9 3.3 3.3
total   7.7   6.6

TBE Gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder BSPP 1CD BSPP 2AB BSPP 2CD
2. Run for 30 minutes at 200V
3. Stain with 3 uL SYBR gold for 2 minutes

Gel Image

File:2013-09-04-DpnIIDigest-BSPP.jpg

Lambda Exonuclease Digestion