Daniel:Notebook/HiResChrPaint/2013-9-30: Difference between revisions
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>Djacobse (Created page with "=Fibroblast Culture= Back to Calendar Split passage 9 cells into 3 culture dishes, also prepped 5 plates fo...") |
>Djacobse |
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3. Aspirated media and added 1 mL fresh DMEM | 3. Aspirated media and added 1 mL fresh DMEM | ||
4. Incubated overnight | 4. Incubated overnight | ||
=FISH ([[Daniel:Notebook/HiResChrPaint/2013-9-27|Started 9/27/2013]])= | |||
==Microscopy== | |||
Performed microscopy on samples from 9/27 using Olympus microscope upstairs. | |||
===Results=== | |||
'''Control''' | |||
{|class="wikitable" | |||
|- | |||
|[[Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png]] | |||
|- | |||
|40x magnification; BF,DAPI,GFP | |||
|} |
Revision as of 19:05, 3 October 2013
Fibroblast Culture
Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.
Prepping cells for hybridization
1. Added 100 uL of cell suspension (after step 5) to center of petri dish 2. Incubated for ~9 hours 3. Aspirated media and added 1 mL fresh DMEM 4. Incubated overnight
FISH (Started 9/27/2013)
Microscopy
Performed microscopy on samples from 9/27 using Olympus microscope upstairs.
Results
Control
Dan_exp_overlay_bfdapigfp_40x_FISH_20130930.png |
40x magnification; BF,DAPI,GFP |