Daniel:Notebook/HiResChrPaint/2013-10-1: Difference between revisions
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>Djacobse (Created page with "=FISH= Back to Calendar ==Slide Preparation== Prepping slides from yesterday. Since I'm not r...") |
>Djacobse |
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===Protocol=== | ===Protocol=== | ||
*Modified from [[Daniel:Protocols/FISH|standard FISH protocol]] | *Modified from [[Daniel:Protocols/FISH#Slide Preparation|standard FISH protocol]], starting at step 5 | ||
1. Rinse slides briefly in 1X PBS | |||
2. Fix slides for 5-15 minutes in 4% (v/v) paraformaldehyde in 1X PBS | |||
3. Rinse briefly with 1 mL 1X PBS | |||
4. Incubate for 5 minutes in 1 mL 2X SSCT | |||
5. Incubate for 5 minutes in 1 mL 2X SSCT + 50% (v/v) formamide | |||
6. Aspirate, and add 1mL of 2X SSCT + 50% (v/v) formamide for storage at 4C | |||
7. Slides are typically best used within 1-2 weeks of creation |
Revision as of 17:16, 1 October 2013
FISH
Slide Preparation
Prepping slides from yesterday. Since I'm not ready to do FISH on them quite yet, I'll just fix them for now.
Protocol
- Modified from standard FISH protocol, starting at step 5
1. Rinse slides briefly in 1X PBS 2. Fix slides for 5-15 minutes in 4% (v/v) paraformaldehyde in 1X PBS 3. Rinse briefly with 1 mL 1X PBS 4. Incubate for 5 minutes in 1 mL 2X SSCT 5. Incubate for 5 minutes in 1 mL 2X SSCT + 50% (v/v) formamide 6. Aspirate, and add 1mL of 2X SSCT + 50% (v/v) formamide for storage at 4C 7. Slides are typically best used within 1-2 weeks of creation