Daniel:Notebook/HiResChrPaint/2013-10-3: Difference between revisions
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>Djacobse |
>Djacobse |
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The control sample worked well enough, it seems like there is plenty of signal but it isn't in any particular place. The experiment group, however, looked terrible, as it looked like there were no cells anywhere on the dish. Further, there were scratches everywhere, distorting the view. I'll have to make sure on future samples that the cells, after fixation, look healthy and plentiful. | The control sample worked well enough, it seems like there is plenty of signal but it isn't in any particular place. The experiment group, however, looked terrible, as it looked like there were no cells anywhere on the dish. Further, there were scratches everywhere, distorting the view. I'll have to make sure on future samples that the cells, after fixation, look healthy and plentiful. | ||
=V6S3/V4S1 Probe Production (Amino primers)= | |||
Since I'm out of V6S3 and V4S1 I'm going to make some more. | |||
==qPCR Amplification== |
Revision as of 21:06, 3 October 2013
FISH (Started 10/01/2013)
Microscopy
Used the Olympus Microscope upstairs to view the most recent round of FISH slides.
Results
Control (BSPP-2)
File:Overlay con 10x FISH2013 10 03.png | File:Overlay con 40x FISH2013 10 03.png |
10x magnification; BF,DAPI,TXRED | 40x magnification; BF,DAPI,TXRED |
Experiment (V6S1+V6S3)
File:Overlay exp 20x FISH 2013 10 03.png |
20x magnification; BF,DAPI,TXRED |
Discussion
The control sample worked well enough, it seems like there is plenty of signal but it isn't in any particular place. The experiment group, however, looked terrible, as it looked like there were no cells anywhere on the dish. Further, there were scratches everywhere, distorting the view. I'll have to make sure on future samples that the cells, after fixation, look healthy and plentiful.
V6S3/V4S1 Probe Production (Amino primers)
Since I'm out of V6S3 and V4S1 I'm going to make some more.