Hosuk:LabNotes/2013-10-9: Difference between revisions
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*488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5) | *488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5) | ||
*20x obj., zoom = 1.00, Pinhole = 2.00 | *20x obj., zoom = 1.00, Pinhole = 2.00 | ||
*Resolution format : 2048 x 2048 Format | *Resolution format : 2048 x 2048 Format (8192 x 8192 --> Pixel size : 70.96nm x 70.96nm) | ||
*Field of view : 581um x 581 um | *Field of view : 581um x 581 um | ||
*Pixel size : 284nm x 284nm | *Pixel size : 284nm x 284nm |
Revision as of 21:35, 9 October 2013
1st Rolonies and ACTB Gene detection in 1st Rolonies
- Tile scan, 2k x 2k resolution images with Confocal
Parameters
- 488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5)
- 20x obj., zoom = 1.00, Pinhole = 2.00
- Resolution format : 2048 x 2048 Format (8192 x 8192 --> Pixel size : 70.96nm x 70.96nm)
- Field of view : 581um x 581 um
- Pixel size : 284nm x 284nm
- PMT1 (ATTO488) : Gain 670V, Offset -2.00%
- PMT2 (Cy5) : Gain 550V, Offset -2.00%
- Z stack : step size : 0.54um, Total 10.87um scan (21 images)
- Tile size = 8 x 6
- Total scanning time : 3hr. 10min