Hosuk:LabNotes/2013-10-19: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Rolonies with fresh cells (96well plate)=== ====Process==== *Start (10/17) *End RCA : 1:30pm 10/19 *...") |
>Hosuki78 No edit summary |
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*End RCA : 1:30pm 10/19 | *End RCA : 1:30pm 10/19 | ||
*... | *... | ||
====Result==== | |||
*Not good : there are rolonies, but almost no ACTB | |||
*Cells in 6 out of 8 wells don’t look good, they were detached, less cells in wells.. WHY? Something happened when fixing? | |||
*Cells were aggregated or became chunk… need to mix when split | |||
*Fixing two step : 37% Formalin 10% Formalin | |||
*The very first protocol was two step fixing, but Jonathan has been doing one step (10% Formalin), and in their manuscript as well. | |||
*But… I want to try two step fixing and compare to one step fixing… |
Revision as of 19:14, 20 October 2013
Rolonies with fresh cells (96well plate)
Process
- Start (10/17)
- End RCA : 1:30pm 10/19
- ...
Result
- Not good : there are rolonies, but almost no ACTB
- Cells in 6 out of 8 wells don’t look good, they were detached, less cells in wells.. WHY? Something happened when fixing?
- Cells were aggregated or became chunk… need to mix when split
- Fixing two step : 37% Formalin 10% Formalin
- The very first protocol was two step fixing, but Jonathan has been doing one step (10% Formalin), and in their manuscript as well.
- But… I want to try two step fixing and compare to one step fixing…