Hosuk:LabNotes/2013-10-22: Difference between revisions

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>Hosuki78
(Created page with "===Rolonies with fresh cells - MatTek dish=== *Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate. ====Process==== *2 dishes *RT :...")
 
>Hosuki78
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**Of course, the number and density of rolonies are still not as many as the sample 06/23...
**Of course, the number and density of rolonies are still not as many as the sample 06/23...


*Doing Confocal
*Doing 1 location as well as tile scanning
**Doing 1 location as well as tile scanning
**Now Confocal is doing tile scanning...
 





Revision as of 23:38, 22 October 2013

Rolonies with fresh cells - MatTek dish

  • Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.

Process

  • 2 dishes
  • RT : 8:30 pm
  • CircLigase : 10/21 12:00 pm
  • End RCA : 10/22 13:00pm
  • Imaging


Result

  • Good, many rolonies, and more ACTB than before.
    • Of course, the number and density of rolonies are still not as many as the sample 06/23...
  • Doing 1 location as well as tile scanning
    • Now Confocal is doing tile scanning...