Hosuk:LabNotes/2013-10-22: Difference between revisions
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>Hosuki78 (Created page with "===Rolonies with fresh cells - MatTek dish=== *Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate. ====Process==== *2 dishes *RT :...") |
>Hosuki78 No edit summary |
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**Of course, the number and density of rolonies are still not as many as the sample 06/23... | **Of course, the number and density of rolonies are still not as many as the sample 06/23... | ||
*Doing 1 location as well as tile scanning | |||
**Now Confocal is doing tile scanning... | |||
Revision as of 23:38, 22 October 2013
Rolonies with fresh cells - MatTek dish
- Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.
Process
- 2 dishes
- RT : 8:30 pm
- CircLigase : 10/21 12:00 pm
- End RCA : 10/22 13:00pm
- Imaging
Result
- Good, many rolonies, and more ACTB than before.
- Of course, the number and density of rolonies are still not as many as the sample 06/23...
- Doing 1 location as well as tile scanning
- Now Confocal is doing tile scanning...
- Some of pictures...
- location 1 : 1st Rolonies, ACTB
- location 2 : 1st Rolonies, ACTB