Daniel:Notebook/HiResChrPaint/2013-11-16: Difference between revisions

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>Djacobse
(Created page with "=Positive Control 2 (V6S3 & V6S4, Started 11/15/2013)= Back to Calendar ==Lambda Exonuclease D...")
 
>Djacobse
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|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
|style="font-weight:bold" height="15" | ng/uL
| align="right" | 169.9
| align="center" | 169.9
| align="right" | 180
| align="center" | 180
| align="right" | 169.4
| align="center" | 169.4
| align="right" | 180.1
| align="center" | 180.1


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 25 uL
|style="font-weight:bold" height="15" | ug in 25 uL
| align="right" | 4.2
| align="center" | 4.2
| align="right" | 4.5
| align="center" | 4.5
| align="right" | 4.2
| align="center" | 4.2
| align="right" | 4.5
| align="center" | 4.5


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | uL added
|style="font-weight:bold" height="15" | uL added
| align="right" | 25
| align="center" | 25
| align="right" | 25
| align="center" | 25
| align="right" | 25
| align="center" | 25
| align="right" | 25
| align="center" | 25


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="30" | uL 10x buffer
|style="font-weight:bold" height="30" | uL 10x buffer
| align="right" | 5
| align="center" | 5
| align="right" | 5
| align="center" | 5
| align="right" | 5
| align="center" | 5
| align="right" | 5
| align="center" | 5


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="45" | uL Lambda Exonuclease (5U/uL)
|style="font-weight:bold" height="45" | uL Lambda Exonuclease (5U/uL)
| align="right" | 3
| align="center" | 3
| align="right" | 3
| align="center" | 3
| align="right" | 3
| align="center" | 3
| align="right" | 3
| align="center" | 3


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | uL nfH2O
|style="font-weight:bold" height="15" | uL nfH2O
| align="right" | 17
| align="center" | 17
| align="right" | 17
| align="center" | 17
| align="right" | 17
| align="center" | 17
| align="right" | 17
| align="center" | 17


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | total
|style="font-weight:bold" height="15" | total
| align="right" | 50
| align="center" | 50
| align="right" | 50
| align="center" | 50
| align="right" | 50
| align="center" | 50
| align="right" | 50
| align="center" | 50


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Revision as of 01:38, 16 November 2013

Positive Control 2 (V6S3 & V6S4, Started 11/15/2013)

Back to Calendar

Lambda Exonuclease Digestion

1. Reaction table
Sample V6S3AB V6S3CD V6S4AB V6S4CD
ng/uL 169.9 180 169.4 180.1
ug in 25 uL 4.2 4.5 4.2 4.5
uL added 25 25 25 25
uL 10x buffer 5 5 5 5
uL Lambda Exonuclease (5U/uL) 3 3 3 3
uL nfH2O 17 17 17 17
total 50 50 50 50
2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C
3. Pool samples
4. ssDNA column purification, elute with 20 uL nfH2O
5. Measure Concentration in Nanodrop