AlanFung:Protocol/EZ DNA Methylation-Direct: Difference between revisions

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[[Media:EZ_DNA_Methylation_Direct_Kit_Protocol.pdf|EZ_DNA_Methylation_Direct_Kit_Protocol PDF]]
[[Media:EZ_DNA_Methylation_Direct_Kit_Protocol.pdf|EZ_DNA_Methylation_Direct_Kit_Protocol PDF]]
==Overview==
*Sample preparation
*Sample Digestion with Proteinase K
*Bisulfite Conversion of DNA
==Protocol==
*Sample Preparation
*Adherent cells
*Suspension cells
*Sample Digestion with Proteinase K
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''2000 cells'''
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''
|-
| M-Digestion Buffer||10ul||13ul
|-
| Sample||up to 9ul||up to 12ul
|-
| Proteinase K||1ul||1ul
|-
| H20||X ul||x ul
|-
| Total Volume||20ul||26 ul
|-
|
|}

Revision as of 20:33, 14 August 2009

Draft

EZ_DNA_Methylation_Direct_Kit_Protocol PDF

Overview

  • Sample preparation
  • Sample Digestion with Proteinase K
  • Bisulfite Conversion of DNA

Protocol

  • Sample Preparation
  • Adherent cells
  • Suspension cells
  • Sample Digestion with Proteinase K
' 2000 cells 100,000 cells
M-Digestion Buffer 10ul 13ul
Sample up to 9ul up to 12ul
Proteinase K 1ul 1ul
H20 X ul x ul
Total Volume 20ul 26 ul