AlanFung:Protocol/EZ DNA Methylation-Direct: Difference between revisions
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[[Media:EZ_DNA_Methylation_Direct_Kit_Protocol.pdf|EZ_DNA_Methylation_Direct_Kit_Protocol PDF]] | [[Media:EZ_DNA_Methylation_Direct_Kit_Protocol.pdf|EZ_DNA_Methylation_Direct_Kit_Protocol PDF]] | ||
==Overview== | |||
*Sample preparation | |||
*Sample Digestion with Proteinase K | |||
*Bisulfite Conversion of DNA | |||
==Protocol== | |||
*Sample Preparation | |||
*Adherent cells | |||
*Suspension cells | |||
*Sample Digestion with Proteinase K | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''2000 cells''' | |||
| align="center" style="background:#f0f0f0;"|'''100,000 cells''' | |||
|- | |||
| M-Digestion Buffer||10ul||13ul | |||
|- | |||
| Sample||up to 9ul||up to 12ul | |||
|- | |||
| Proteinase K||1ul||1ul | |||
|- | |||
| H20||X ul||x ul | |||
|- | |||
| Total Volume||20ul||26 ul | |||
|- | |||
| | |||
|} |
Revision as of 20:33, 14 August 2009
Draft
EZ_DNA_Methylation_Direct_Kit_Protocol PDF
Overview
- Sample preparation
- Sample Digestion with Proteinase K
- Bisulfite Conversion of DNA
Protocol
- Sample Preparation
- Adherent cells
- Suspension cells
- Sample Digestion with Proteinase K
' | 2000 cells | 100,000 cells |
M-Digestion Buffer | 10ul | 13ul |
Sample | up to 9ul | up to 12ul |
Proteinase K | 1ul | 1ul |
H20 | X ul | x ul |
Total Volume | 20ul | 26 ul |