AlanFung:Protocol/EZ DNA Methylation-Direct: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
>Alan6017518
Line 21: Line 21:
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''
| align="center" style="background:#f0f0f0;"|'''100,000 cells'''
|-
|-
| M-Digestion Buffer||10ul||13ul
| M-Digestion Buffer (2X)||10ul||13ul
|-
|-
| Sample||up to 9ul||up to 12ul
| Sample||up to 9ul||up to 12ul

Revision as of 20:34, 14 August 2009

Draft

EZ_DNA_Methylation_Direct_Kit_Protocol PDF

Overview

  • Sample preparation
  • Sample Digestion with Proteinase K
  • Bisulfite Conversion of DNA

Protocol

  • Sample Preparation
  • Adherent cells
  • Suspension cells
  • Sample Digestion with Proteinase K
2000 cells 100,000 cells
M-Digestion Buffer (2X) 10ul 13ul
Sample up to 9ul up to 12ul
Proteinase K 1ul 1ul
H20 X ul x ul
Total Volume 20ul 26 ul