Daniel:Notebook/HiResChrPaint/2014-1-9: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 48: | Line 48: | ||
2. Incubate samples at 37C for '''2''' hours; 20 minute heat shock at 65C | 2. Incubate samples at 37C for '''2''' hours; 20 minute heat shock at 65C | ||
3. [[Daniel:Protocols/ | 3. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], elute with 30 uL EB | ||
4. Measure concentrations in nanodrop | 4. Measure concentrations in nanodrop |
Revision as of 17:53, 9 January 2014
Alu Control (Started 1/08/2014)
DpnII Digestion
I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion.
1. Reaction Table
Sample | ALU2-A | ALU2-B |
ng/uL | 25.1 | 25 |
ug in 30 uL | 0.8 | 0.8 |
uL 10X buffer | 5 | 5 |
uL DpnII (50U/uL) | 1 | 1 |
uL nfH2O | 14 | 14 |
Total | 50 | 50 |
2. Incubate samples at 37C for 2 hours; 20 minute heat shock at 65C 3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop