Daniel:Notebook/HiResChrPaint/2014-1-9: Difference between revisions

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|style="font-weight:bold" height="15" | ug in 30 uL
|style="font-weight:bold" height="15" | ug in 35 uL
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|style="font-weight:bold" height="15" | uL nfH2O
|style="font-weight:bold" height="15" | uL nfH2O
| align="center" | 14
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Revision as of 17:56, 9 January 2014

Alu Control (Started 1/08/2014)

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DpnII Digestion

I'm going to do a test digestion/gel to make sure this works. Since I had such low input I'm going to use 2 samples and pool after the digestion.

1. Reaction Table
Sample ALU2-A ALU2-B
ng/uL 25.1 25
ug in 35 uL 0.9 0.9
uL 10X buffer 5 5
uL DpnII (50U/uL) 1 1
uL nfH2O 9 9
Total 50 50
2. Incubate samples at 37C for 2 hours; 20 minute heat shock at 65C
3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop